[Histonet] RE: negative controls
Martha Ward-Pathology
mward <@t> wakehealth.edu
Fri Aug 17 11:47:21 CDT 2012
We eliminated them on August 1st, except in cases where they are specifically requested. So far we have run <10 negative slides.
Martha Ward, MT (ASCP) QIHC
Manager
Molecular Diagnostics Lab
Medical Center Boulevard \ Winston-Salem, NC 27157
p 336.716.2109 \ f 336.716.5890
mward <@t> wakehealth.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, August 17, 2012 12:36 PM
To: 'Romundstad, Pamela K'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: negative controls
For everyone performing immunos are you not running negative controls now with this new ruling from CAP?
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hornhv <@t> archildrens.org archildrens.org
100 YEARS YOUNG!
JOIN THE PARTY AT
ach100.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Romundstad, Pamela K
Sent: Friday, August 17, 2012 10:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Histonet Digest, Vol 105, Issue 20
Yes, the negative control updated change is in effect as of July 31. 2012. I contacted CAP personally this week and was directed to the revised ANP.22570. It only applies to polymer based detection systems (biotin-free).
Respectfully,
Pamela Romundstad HT, QIHC
Gundersen Lutheran
608-775-3139
________________________________________
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Sent: Thursday, August 16, 2012 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 105, Issue 20
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Today's Topics:
1. protocol for EGFP IHC in frozen rat brains (Bass, Caroline)
2. Re: Two identifiers... (Bob Richmond)
3. RE: Re: Two identifiers... (joelle weaver)
4. RE: Re: Two identifiers... (Michael Mihalik)
5. Negative Control (Pardue, Judith)
6. RE: Special Stain documentation (hymclab)
7. Peloris (Pardue, Judith)
8. RE: RE: Two identifiers... (Pratt, Caroline)
9. RE: RE: Two identifiers... (Tom McNemar)
10. marker specific for mouse fibroblasts (Kim Merriam)
11. RE: RE: Two identifiers... (Pratt, Caroline)
12. RE: marker specific for mouse fibroblasts (Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Wed, 15 Aug 2012 18:07:46 -0400
From: "Bass, Caroline" <cebass <@t> buffalo.edu>
Subject: [Histonet] protocol for EGFP IHC in frozen rat brains
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <ABC535EC-9FCD-45E0-957C-617FA7AA8B53 <@t> buffalo.edu>
Content-Type: text/plain; charset="Windows-1252"
Hello Everyone,
I have some rat brains that should express GFP, the brains were collected fresh and snap frozen in dry ice/isopentane. They have been stored for several months in a -80. I'd like to collect 500 um sections, thaw mount them to a slide an reserve these for mRNA collection. I would then like to take intervening sections to stain for GFP. I have a staining protocol for perfused, formalin fixed, sucrose protected floating brain sections that works fine. I'm a little more concerned about moving to unfixed sections. I know I need to cut everything on a cryostat and I assume that I have to process the thinner sections on the slide instead of free floating. What is the best way to proceed? Here are some specific questions?
1) what thickness should I cut, 50 um works great for my fixed sections.
2) should I "fix" these sections by either paraformaldehyde vapors or dunking in fix? If so, what kind/concentration?
3) could I then proceed as though this were a normal GFP immuno, with DAB stain?
4) should I used plus slides or subbed slides?
5) will the sections stay on without fixation?
Any and all suggestions would be appreciated!
Thanks,
Caroline
------------------------------
Message: 2
Date: Wed, 15 Aug 2012 21:11:12 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Two identifiers...
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH5bHaSnoncK7Zg+04QjD23O0nfo+5T-8FjL71hS6afggQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
If a pathologist end-user may make an observation here:
When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases.
I can't do that with a bar code or a second essentially meaningless number.
This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance.
Bob Richmond
Samurai Pathologist
Maryville TN
------------------------------
Message: 3
Date: Thu, 16 Aug 2012 05:24:56 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] Re: Two identifiers...
To: <rsrichmond <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <SNT135-W523C7297BE0A6C24B5B44BD8B50 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Yes I can see how that would be easier for the Pathologist. Also you certainly fall into "need to know". HIPPA applies for information when it gets electronically transmitted, so not so much the physical slide would be my assumption. Some places just want to be very cautious I guess, and are concerned about associating the name with the number, though you would still need to find a way to access anymore information in the system or papers. I am not saying I agree with this interpretation necessarily, just that is what I have experienced so far, perhaps it is overkill? I know that one issue I see is that when people see a name of someone they think they know in the lab itself, it makes them much more curious and they are tempted to look them up- a friend, neighbor, co-worker. Of course most LIS audit for this, but not only is this very offensive when you hear this person's information reported and discussed out in the lab, it is one of the reasons I support HIPPA generally despite its burdens. And also why I try never to have my own specimens go to a lab I am working at or know anyone. I realize barcodes are not helpful to people doing handling unfortunately, but they do help avoid transcription errors, offer an additional layer to disguise the information from those who don't need it and might be overly curious, and allow tracking for workflow- but many people still prefer human-manual labeling methods.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Wed, 15 Aug 2012 21:11:12 -0400
> From: rsrichmond <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Two identifiers...
>
> If a pathologist end-user may make an observation here:
>
> When I pick up a slide and look to match it with the paperwork in
> front of me, it's a great help if the patient's name is on the slide,
> since names are always easier to read than numbers. Having the
> patient's name on the slide means I'm less likely to mix up two cases.
> I can't do that with a bar code or a second essentially meaningless
> number.
>
> This may however be a situation where good patient care must take a
> back seat to good management practice and good regulator compliance.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Thu, 16 Aug 2012 05:04:17 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Re: Two identifiers...
To: "'Bob Richmond'" <rsrichmond <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000b01cd7b8e$29493b10$7bdbb130$@pathview.com>
Content-Type: text/plain; charset="iso-8859-1"
Bob, I absolutely concur with your thoughts, but in the LIS we offer, the moment you scan the slide, the 'paperwork' displays on the screen automatically, effectively negating the need to match paperwork with the slide.
We also always recommend BIG monitors or even dual monitors to make it easier to see. Imagine the paperwork on one monitor and a 'work' screen on the other monitor.
Michael Mihalik
PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Wednesday, August 15, 2012 9:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Two identifiers...
If a pathologist end-user may make an observation here:
When I pick up a slide and look to match it with the paperwork in front of me, it's a great help if the patient's name is on the slide, since names are always easier to read than numbers. Having the patient's name on the slide means I'm less likely to mix up two cases.
I can't do that with a bar code or a second essentially meaningless number.
This may however be a situation where good patient care must take a back seat to good management practice and good regulator compliance.
Bob Richmond
Samurai Pathologist
Maryville TN
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 16 Aug 2012 04:49:50 -0600
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Negative Control
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14B823F24E628E49BBFAD704E4BAB89ABE9BCD <@t> chimsx03.CHI.catholichealth.net>
Content-Type: text/plain; charset="us-ascii"
At the first of August Richard Cartun posted that CAP had a new negative control policy. Does anyone know if it is already in effect and if it would apply to up coming inspections.
Judith Pardue
Histology Supervisor
Memorial Hospital
Chattanooga, Tn.
Judith_Pardue <@t> memorial.org
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Message: 6
Date: Thu, 16 Aug 2012 07:51:41 -0500
From: hymclab <hymclab.hymclab <@t> ministryhealth.org>
Subject: RE: [Histonet] Special Stain documentation
To: "'Weems, Joyce K.'" <joyce.weems <@t> emoryhealthcare.org>,
"'laurie <@t> blufrogpath.com'" <laurie <@t> blufrogpath.com>, Histonet post
<histonet <@t> lists.utsouthwestern.edu>
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<EF3001233998854390C692D19B058F0F70FECA4B60 <@t> EXMHCMBX01VS.ministryhealth.net>
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For H&E's, a log goes to the Pathologist with the most slides for the day and he checks if the technical quality of the slides is good or not and initials. The lead tech also initials.
For Special Stains, we have a QC log that we document stains performed on daily and the results of the stains. The Pathologists also document along with special stain results that "Controls reacted appropriately" in the report.
Dawn
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, August 13, 2012 5:06 PM
To: 'Laurie <@t> blufrogpath.com'; Histonet post
Subject: RE: [Histonet] Special Stain documentation
Our techs document that the controls work. My dream would be that the pathologist would document in the report with a phrase something like "Controls stain appropriately".
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie <@t> blufrogpath.com
Sent: Monday, August 13, 2012 5:35 PM
To: Histonet post
Subject: [Histonet] Special Stain documentation
How do others document the results of routine special stain controls to be acceptable before reporting patient results (CAP checklist item ANP.21395)? Do the histotechs document the results or do the pathologists - or both?? If your pathologists document the results, how do they document them?
Thanks,
Laurie Colbert
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Message: 7
Date: Thu, 16 Aug 2012 07:17:20 -0600
From: "Pardue, Judith" <Judith_Pardue <@t> memorial.org>
Subject: [Histonet] Peloris
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14B823F24E628E49BBFAD704E4BAB89ABE9C2F <@t> chimsx03.CHI.catholichealth.net>
Content-Type: text/plain; charset="us-ascii"
Has anyone had problems with Peloris processors shuting down after a power outage. Both our processors shut down when the power went off last night and they were connected to a USP and
plugged into an emergency outlet. This is the third time this has happened and we have had to have a service tech from Leica come out.
Judith Pardue
Histology Supervisor
Memorial Hospital
Judith_Pardue <@t> memorial.org
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Message: 8
Date: Thu, 16 Aug 2012 09:46:54 -0400
From: "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu>
Subject: RE: [Histonet] RE: Two identifiers...
To: "Ron" <pathrm35 <@t> comcast.net>, <pamarcum <@t> uams.edu>,
<tmcnemar <@t> lmhealth.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EFA78D6D81C0F348B8FA2880AE376CC346E717 <@t> uphmasphi015.UPHS.PENNHEALTH.PRV>
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It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...
We do the same as Pam with the patient name and case number
Sent from my Verizon Wireless 4G LTE smartphone
"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:
>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
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Message: 9
Date: Thu, 16 Aug 2012 09:50:45 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] RE: Two identifiers...
To: "'Pratt, Caroline'" <Caroline.Pratt <@t> uphs.upenn.edu>, Ron
<pathrm35 <@t> comcast.net>, "pamarcum <@t> uams.edu" <pamarcum <@t> uams.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<E9A90E28259D2F4E84308C5E8EA8F7B4011F577646E5 <@t> lmhs-exchange.lmhealth.org>
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I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: Pratt, Caroline [mailto:Caroline.Pratt <@t> uphs.upenn.edu]
Sent: Thursday, August 16, 2012 9:47 AM
To: Ron; pamarcum <@t> uams.edu; Tom McNemar; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...
It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...
We do the same as Pam with the patient name and case number
Sent from my Verizon Wireless 4G LTE smartphone
"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:
>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
>_______________________________________________
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>confidential and privileged information. Any unauthorized review, use,
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>recipient, please contact the sender by reply e-mail and destroy all
>copies of the original message.
>
>
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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
------------------------------
Message: 10
Date: Thu, 16 Aug 2012 07:01:11 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] marker specific for mouse fibroblasts
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1345125671.55062.YahooMailNeo <@t> web130105.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello All!
I am looking for a marker that is highly specific for mouse fibroblasts.? I have tried several markers, but none of them seem to be specific for ONLY fibroblasts.
Kim
?
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
------------------------------
Message: 11
Date: Thu, 16 Aug 2012 10:02:07 -0400
From: "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu>
Subject: RE: [Histonet] RE: Two identifiers...
To: "Tom McNemar" <TMcNemar <@t> lmhealth.org>, "Ron"
<pathrm35 <@t> comcast.net>, <pamarcum <@t> uams.edu>,
<histonet <@t> lists.utsouthwestern.edu>
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<EFA78D6D81C0F348B8FA2880AE376CC346E71A <@t> uphmasphi015.UPHS.PENNHEALTH.PRV>
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I am so sorry to cause confusion, I am referring to specimen bottles, my apologies.
-----Original Message-----
From: Tom McNemar [mailto:TMcNemar <@t> lmhealth.org]
Sent: Thursday, August 16, 2012 9:51 AM
To: Pratt, Caroline; Ron; pamarcum <@t> uams.edu; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...
I am a little confused. Are you referring to specimen bottles? I was inquiring about what identifiers are being used on printed cassettes.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: Pratt, Caroline [mailto:Caroline.Pratt <@t> uphs.upenn.edu]
Sent: Thursday, August 16, 2012 9:47 AM
To: Ron; pamarcum <@t> uams.edu; Tom McNemar; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Two identifiers...
It doesn't meet criteria for TJC, we used that for years and it was acceptable until our last survey. We were written up for it and had to send labeling instructions to all of our submitting clinicians and conduct bottle audits to evidence that 90% of all bottles had patient full name (last and first), DOB and site along with the accession number. It is a tremendous ongoing effort, but we are there now.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ron
Sent: Wednesday, August 15, 2012 12:39 PM
To: pamarcum <@t> uams.edu; tmcnemar <@t> lmhealth.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Two identifiers...
We do the same as Pam with the patient name and case number
Sent from my Verizon Wireless 4G LTE smartphone
"Marcum, Pamela A" <PAMarcum <@t> uams.edu> wrote:
>We use the specimen or accessioning number and the patient name as the two identifiers and that seems to meet the criteria. We use the Thermo cassette writer and it transfers the information to the slides.
>
>Pam Marcum
>UAMS
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom
>McNemar
>Sent: Wednesday, August 15, 2012 10:03 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Two identifiers...
>
>Hello all,
>
>For those of you using cassette and slide labelers....
>Does your system permit you to print the accession number, patient name, and DOB on the face of the cassette? Are you using the specimen number and name as your two identifiers?
>
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Health Systems
>(740) 348-4163
>(740) 348-4166
>tmcnemar <@t> lmhealth.org<mailto:tmcnemar <@t> lmhealth.org>
>www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Applica
>tion%20Data\Microsoft\Signatures\www.LMHealth.org>
>
>________________________________
>This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
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------------------------------
Message: 12
Date: Thu, 16 Aug 2012 08:51:32 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] marker specific for mouse fibroblasts
To: 'Kim Merriam' <kmerriam2003 <@t> yahoo.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE0162D07DAD12 <@t> SBS2K8.premierlab.local>
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Kim
There are several markers out there for fibroblasts, I think we have used FSP-1 for mouse. I know we have used several different markers for different species such as pro collagen type I and prolyl-4-hydroxylase and possibly others we worked on a bunch of wound healing models in both rat and human a few years back, but I don't think we did much on mouse. Acris has a lot of information on their website. In a different e-mail I'll forward you something I pulled off the Acris site on fibroblasts. Good Luck.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Thursday, August 16, 2012 8:01 AM
To: Histonet
Subject: [Histonet] marker specific for mouse fibroblasts
Hello All!
I am looking for a marker that is highly specific for mouse fibroblasts. I have tried several markers, but none of them seem to be specific for ONLY fibroblasts.
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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End of Histonet Digest, Vol 105, Issue 20
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