[Histonet] RE: Eosin

[Burton, Lynn]

We have used a mixture of Eosin Y and Phloxine B with acetic acid as follows:
780mL 95 %ETOH
100mL Eosin
10mL Phloxine B
4mL glacial acetic acid
We seem to be consistent. We have used this for 17 years.
 
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie [Valerie.Hannen <@t> parrishmed.com]
Sent: Thursday, August 09, 2012 10:23 AM
To: histonet
Subject: [Histonet] Eosin

Hi Folks...I am hoping you all give me a little help.  Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one

day to the next...one day it looks great...the next it is weak!!

I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the  alcohols after Eosin are the proper concentrations....3)

 reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing

 steps. 5) I have checked the pH of the water as well.

Any help and suggestions would greatly appreciated!!

Thanks Gang!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com







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