[Histonet] RE: Eosin

[Geoff McAuliffe]

Wanda makes an excellent point, thinner sections have less contrast.

Geoff

On 8/9/2012 2:50 PM, Wanda.Smith <@t> HCAhealthcare.com wrote:
> How about the thickness of the tissue?  Once we were cutting skins at 3 microns and the pathologist complained the Eosin was too light so we started cutting them at 4-5 microns.
>
>
> WANDA G. SMITH, HTL(ASCP)HT
> Pathology Supervisor
> TRIDENT MEDICAL CENTER
> 9330 Medical Plaza Drive
> Charleston, SC  29406
> 843-847-4586
> 843-847-4296 fax
>
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie
> Sent: Thursday, August 09, 2012 11:23 AM
> To: histonet
> Subject: [Histonet] Eosin
>
> Hi Folks...I am hoping you all give me a little help.  Our Pathologists are complaining about our Eosin on the H&E's being weak. The funny thing is, is that it can go one
>
> day to the next...one day it looks great...the next it is weak!!
>
> I have already done some experimenting with...1) time tissue spends in Eosin.... 2) making sure that the  alcohols after Eosin are the proper concentrations....3)
>
>   reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing
>
>   steps. 5) I have checked the pH of the water as well.
>
> Any help and suggestions would greatly appreciated!!
>
> Thanks Gang!!
>
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.hannen <@t> parrishmed.com
>
>
>
>
>
>
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-- 
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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