[Histonet] Ki67 antibody to stain Mouse tissue

[Eva Permaul]

Just to clarify. Are you using it on mouse tissues? I am only asking
because the Thermo Scientific/Labvision/Neomarkers data sheet says it has
only been verified on Human tissues.
Secondly had anyone used it on mouse gut. Does it stain in the bottom of
the crypts or at the top? I am asking because I have also tried a different
antibody from Leica but got staining at the top of the crypts not at the
bottom.

Thank you to everyone for all of your valuable suggestions and advice,
Eva Permaul
Georgetown University

On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) <
joost.bruijntjes <@t> tno.triskelion.nl> wrote:

> Hi Eva
>
> I've used an antibody from Labvision /Neomarkers. It is a rabbit
> monoclonal; catalog number: RM-9106.
>
> Joost
>
> -----Oorspronkelijk bericht-----
> Van: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] Namens Eva Permaul
> Verzonden: maandag 6 augustus 2012 20:44
> Aan: histonet
> Onderwerp: [Histonet] Ki67 antibody to stain Mouse tissue
>
> Hello,
> I am looking for a Ki67 to stain mouse tissues. We have been using Dako
> M2749 but just found out it has been discontinued. Could anyone suggest a
> good alternative?
> Thank you all for any help,
> Eva Permaul
> Georgetown University
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