[Histonet] Ki67 antibody to stain Mouse tissue

Colleen Forster cforster <@t> umn.edu
Mon Aug 6 15:57:53 CDT 2012


Thanks Jan, that would be the same one I have and it works great!

Colleen Forster
U of MN


On 8/6/2012 2:59 PM, Jan Shivers wrote:
> We have had success with Lab Vision's rabbit polyclonal on mouse tissue
> (catalog # RB-9043), utilizing HIER for antigen retrieval.
>
> Jan Shivers
> U of MN Vet Diag Lab
>
> On Mon, Aug 6, 2012 at 1:48 PM, Eva Permaul <eca9 <@t> georgetown.edu> wrote:
>
>> We are staining straight mouse tissue.
>>
>> On Mon, Aug 6, 2012 at 2:46 PM, Sarah Dysart <sdysart <@t> mirnarx.com> wrote:
>>
>>> Are you staining human xenografts in mice or straight out mouse tissue?
>>   I
>>> get all mine from abcam.  I am currently staining xenografts so I am
>> using
>>> a human specific one, but I think it might be good for mouse too?
>>>
>>> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
>>> Histotechnologist
>>> Mirna Therapeutics
>>> 2150 Woodward Street
>>> Suite 100
>>> Austin, Texas  78744
>>> (512)901-0900 ext. 6912
>>>
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
>>> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva Permaul
>>> Sent: Monday, August 06, 2012 1:44 PM
>>> To: histonet
>>> Subject: [Histonet] Ki67 antibody to stain Mouse tissue
>>>
>>> Hello,
>>> I am looking for a Ki67 to stain mouse tissues. We have been using Dako
>>> M2749 but just found out it has been discontinued. Could anyone suggest a
>>> good alternative?
>>> Thank you all for any help,
>>> Eva Permaul
>>> Georgetown University
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>>>
>>>
>>>
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