[Histonet] GMS on toenails
Santiago, Albert
Albert.Santiago <@t> uphs.upenn.edu
Mon Apr 23 07:23:33 CDT 2012
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, April 19, 2012 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GMS on Toenail
Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
Hello Allison, we do PAS and GMS on toenails just about every day since we have a nail clinic in our department. First we dip our slides in Stay On solution (SurgiPath) and allow them to dry then we soak the faced nail block in 4% Ammonium Hydroxide for 45-60 minutes before we cut a ribbon and place on slides. We let the slides sit in a 70 degree oven for about 45 minutes, we Deparaffinize, hydrate to DH2O and we stain on the Artisan Special Stain stainer. Works every time. If it keeps falling off you might need to do it manually. Good Luck
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, April 21, 2012 1:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 101, Issue 28
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Today's Topics:
1. Re: Histonet Digest, Vol 101, Issue 27 (Eric Tambutte)
2. Re: Pinning Specimens (Bob Richmond)
3. Immunofluorescence (Thotakura, Anil Kumar)
4. RE: GMS on Toenail (Britton, Josette C)
5. Leica Peloris Processor (Joe W. Walker, Jr.)
6. Re: Leica Peloris Processor (Richard Cartun)
7. Re: Leica Peloris Processor (Patrick Laurie)
8. Re: Histonet Digest, Vol 101, Issue 27 (Amos Brooks)
----------------------------------------------------------------------
Message: 1
Date: Fri, 20 Apr 2012 19:03:08 +0200
From: Eric Tambutte <etambutte <@t> centrescientifique.mc>
Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1083480164 <@t> s15272523.onlinehome-server.info>
Bonjour,
Je suis absent du laboratoire jusqu'au jeudi 03 mai 2012. Je vous répondrai le plus rapidement possible.
Eric Tambutté
Thank you for your mail. I will be out of office till May 03rd 2012.
I will respond to your e-mail as soon as possible.
Thank you for your understanding.
Best regards
Eric Tambutté
------------------------------
Message: 2
Date: Fri, 20 Apr 2012 13:20:15 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Pinning Specimens
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOKsRH5gcGSm6hesAYW40JR0LfxSSrE_f42ZE0Hde1PVE0eemA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
About pinning specimens so they fix flat: Margaret Horne notes the use
of dental wax. I've used it to make small "boats" for the rather
exacting procedure of pinning muscle biopsy specimens for electron
microscopy. Here a 1 to 2 mm bundle of longitudinal fibers has to be
gently stretched to its resting length, pinned in the "boat", and
promptly fixed in glutaraldehyde.
One technologist noted that she had a young pathologist who was "wet
behind the ears" and wanted to pin specimens, and was looking to the
older pathologists to bring him in line to The Way We've Always Done
It. I try to remain "wet behind the ears", though after nearly 50
years of occasionally pinning specimens I find myself a little dry.
We continue to lose skills in the gross room. Today I'm working with a
service that has almost no ability to handle calcified tissue - I need
to go to the hardware store and buy a hacksaw to replace the worn-out
Satterlee saw, which I think was gathered up off a Civil War
battlefield.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 3
Date: Fri, 20 Apr 2012 18:01:17 +0000
From: "Thotakura, Anil Kumar" <a.thotakura <@t> imperial.ac.uk>
Subject: [Histonet] Immunofluorescence
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CBB762FA.14AA6%a.thotakura <@t> imperial.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Dear All,
Can some one please send the protocol for immunofluorescence, I want to stain for FITC CD45.1 on mice spleens.
Thank you very much for your help.
Many Thanks,
Anil kumar.
------------------------------
Message: 4
Date: Fri, 20 Apr 2012 14:54:29 -0400
From: "Britton, Josette C" <JCBRITTON <@t> Cheshire-Med.COM>
Subject: RE: [Histonet] GMS on Toenail
To: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F644CD64B2313F43BB3D2496B454CDAC09A35674 <@t> CMC-EX01.cheshire-med.com>
Content-Type: text/plain; charset="us-ascii"
The way we got around that problem was to do a manual PAS stain instead!
Josie Britton HT
Cheshire Medical Center
Keene NH
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Thursday, April 19, 2012 3:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GMS on Toenail
Hello to all in histoland. We have a stubborn toenail that keeps coming
off when we try to do a GMS stain on the ventana machine. Any
suggestions on how to keep the section on the slide during the staining
procedure.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas
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Message: 5
Date: Fri, 20 Apr 2012 19:47:13 +0000
From: "Joe W. Walker, Jr." <joewalker <@t> rrmc.org>
Subject: [Histonet] Leica Peloris Processor
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Hello all,
I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results.
Looking forward to responses,
Joe W. Walker, Jr. SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
Phone: 802.747.1790 Fax:802.747.6525
NEW EMAIL: joewalker <@t> rrmc.org<mailto:joewalker <@t> rrmc.org>
www.rrmc.org<http://www.rrmc.org>
Our Vision: To be the Best Healthcare System in New England
Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence
This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail.
Thank You
------------------------------
Message: 6
Date: Fri, 20 Apr 2012 16:07:16 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Leica Peloris Processor
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Jr. Joe W. Walker"
<joewalker <@t> rrmc.org>
Message-ID: <4F9189B4.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
In my experience, no. Our current IHC/ISH is as good, if not better than what we were getting with our former processors.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Joe W. Walker, Jr." <joewalker <@t> rrmc.org> 4/20/2012 3:47 PM >>>
Hello all,
I am new to your listserve. I am wondering if anyone has experience with the Leica Peloris processor. Specifically, has anyone noticed if the higher processing temperatures has affected any IHC staining results or FISH results.
Looking forward to responses,
Joe W. Walker, Jr. SCT(ASCP)CM
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
Phone: 802.747.1790 Fax:802.747.6525
NEW EMAIL: joewalker <@t> rrmc.org<mailto:joewalker <@t> rrmc.org>
www.rrmc.org<http://www.rrmc.org>
Our Vision: To be the Best Healthcare System in New England
Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence
This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail.
Thank You
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Fri, 20 Apr 2012 13:29:30 -0700
From: Patrick Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] Leica Peloris Processor
To: "Joe W. Walker, Jr." <joewalker <@t> rrmc.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAKEyg-0babdOMrHwET04LxwkBwe9bj51vPO6oz7ehs=TWa5R=A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We have 4 peloris processors, run many IHC and FISH stains, none of them
have had any negative effects due to the increased temperatures. The
increased temps aren't that high, 45 degrees for all the liquids (we use
xylene) and 65 degrees for the paraffins.
Good luck
Patrick
On Fri, Apr 20, 2012 at 12:47 PM, Joe W. Walker, Jr. <joewalker <@t> rrmc.org>wrote:
> Hello all,
>
> I am new to your listserve. I am wondering if anyone has experience with
> the Leica Peloris processor. Specifically, has anyone noticed if the
> higher processing temperatures has affected any IHC staining results or
> FISH results.
>
> Looking forward to responses,
>
> Joe W. Walker, Jr. SCT(ASCP)CM
> Anatomical Pathology Manager
> Rutland Regional Medical Center
> 160 Allen Street, Rutland, VT 05701
> Phone: 802.747.1790 Fax:802.747.6525
> NEW EMAIL: joewalker <@t> rrmc.org<mailto:joewalker <@t> rrmc.org>
> www.rrmc.org<http://www.rrmc.org>
>
> Our Vision: To be the Best Healthcare System in New England
> Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet
> Recognition(r) and the Governor's Award for Performance Excellence
>
> This message (and any included attachments) is from Rutland Regional
> Health Services and is intended only for the addressee(s). The information
> contained herein may include privileged or otherwise confidential
> information. Unauthorized review, forwarding, printing, copying,
> distributing, or using such information is strictly prohibited and may be
> unlawful. If you received this message in error, or have reason to believe
> you are not authorized to receive it, please promptly delete this message
> and notify the sender by e-mail.
>
> Thank You
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com
------------------------------
Message: 8
Date: Fri, 20 Apr 2012 17:46:16 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 101, Issue 27
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC95ki908wAAmhw-Ny=WThJ9mBAk4QG6x1LKfikPCdFnsr3CUQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
Strange issue with your Ki-67. I don't know what would do that unless
it was non-specific background. Is it nuclear or cutoplasmic?
If you are working in mice or other animals you could try labeling
them with BRDu then detecting it with an anti BRDu antibody. Humans don't
like being injected with BRDu then being sacrifices but lawyers do. Also
Cleaved Caspase 3 works nicely and is probably more specific to apoptosis
than Tunel which also picks up necrosis.
Have a great weekend,
Amos
On Fri, Apr 20, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:
> Message: 9
> Date: Thu, 19 Apr 2012 15:07:43 -0500
> From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
> Subject: [Histonet] proliferation and apoptosis
> To: "histonet-request <@t> lists.utsouthwestern.edu"
> <histonet-request <@t> lists.utsouthwestern.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <
> C1BA93040C6B9A4A8D84878F93FEC36A0AB7D0E9F1 <@t> CMHEXCC01MBX.childrensmemorial.org
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi histonetters,
>
> I am looking for the good markers to detect (separately) proliferation and
> apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic
> bodies as well as proliferated cells; and the tunnel assay shows both
> apoptotic body and proliferation.
>
> Any suggestions for the FFPE tissue and the HRP protocol are appreciated.
>
> Thanks in advance,
> Naira
>
------------------------------
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