[Histonet] proliferation and apoptosis

[Jackie O'Connor]

Ki67 does not stain apoptotic cells. Why do you have that impression?  Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation.   I've used Ki67 for proliferation  and Caspase 3 for apoptosis routinely in cancer research for years.

Jackie


-----Original Message-----
From: Margaryan, Naira <NMargaryan <@t> childrensmemorial.org>
To: histonet-request <histonet-request <@t> lists.utsouthwestern.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, Apr 19, 2012 3:09 pm
Subject: [Histonet] proliferation and apoptosis


Hi histonetters,

I am looking for the good markers to detect (separately) proliferation and 
apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic 
bodies as well as proliferated cells; and the tunnel assay shows both apoptotic 
body and proliferation.

Any suggestions for the FFPE tissue and the HRP protocol are appreciated.

Thanks in advance,
Naira

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