[Histonet] proliferation and apoptosis
b427297 <@t> aol.com
Thu Apr 19 16:23:09 CDT 2012
Ki67 does not stain apoptotic cells. Why do you have that impression? Caspase-3 is a great marker for apoptotic cells. TUNEL will show apoptosis as well as necrosis, but not proliferation. I've used Ki67 for proliferation and Caspase 3 for apoptosis routinely in cancer research for years.
From: Margaryan, Naira <NMargaryan <@t> childrensmemorial.org>
To: histonet-request <histonet-request <@t> lists.utsouthwestern.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, Apr 19, 2012 3:09 pm
Subject: [Histonet] proliferation and apoptosis
I am looking for the good markers to detect (separately) proliferation and
apoptosis of cells in tumor sections. Unfortunately, KI-67 stains apoptotic
bodies as well as proliferated cells; and the tunnel assay shows both apoptotic
body and proliferation.
Any suggestions for the FFPE tissue and the HRP protocol are appreciated.
Thanks in advance,
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