[Histonet] Steiner stain
Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Apr 18 11:57:07 CDT 2012
I imagine that you also ran a (+) control. If in the (+) control the organisms are well stained, just remove the coverslip, counterstain again and cover again.
--- On Wed, 4/18/12, Fran Pearsall <fpearsa <@t> clemson.edu> wrote:
From: Fran Pearsall <fpearsa <@t> clemson.edu>
Subject: [Histonet] Steiner stain
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, April 18, 2012, 11:31 AM
The counter stain faded on my slides. I've never had that happen before, but they were left overnight in zylene before being cover-slipped. Can I go back and remove the cover slip, which step would I back up to regain the staining? Or, do I need to completely start the stain again? We use the micro-wave method using a box kit from Sigma, the Accumate H2 100 . I wonder if I need to re-make the reducing solution, I was trying to see if I can save using another hydroquinone tablet?
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