[Histonet] Steiner stain

[Rene J Buesa]

I imagine that you also ran a (+) control. If in the (+) control the organisms are well stained, just remove the coverslip, counterstain again and cover again.
René J.

--- On Wed, 4/18/12, Fran Pearsall <fpearsa <@t> clemson.edu> wrote:


From: Fran Pearsall <fpearsa <@t> clemson.edu>
Subject: [Histonet] Steiner stain
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, April 18, 2012, 11:31 AM


Hi  Everyone,

The counter stain faded on my slides.  I've never had that happen before, but they were left overnight in zylene before being cover-slipped.  Can I go back and remove the cover slip,  which step would I back up to regain the staining?  Or, do I need to completely start the stain again?  We use the micro-wave method using a box kit from Sigma, the Accumate H2 100 .  I wonder if I need to re-make  the reducing solution, I was trying to see if I can save using another hydroquinone tablet?

Thanks


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