[Histonet] Cassette labeling problem

Blazek, Linda lblazek <@t> digestivespecialists.com
Mon Apr 9 10:19:44 CDT 2012

It may be your cassette and not your marker.  That was the problem we encountered awhile ago.  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Monday, April 09, 2012 11:17 AM
To: Histonet
Subject: [Histonet] Cassette labeling problem

Hi All:

Lately I have been having problems with the ink fading-and/or simply coming off in pieces-from our processing cassettes.
I use Securline Marker 2/Superfrost pens.
The writing seems to hold fine even if the cassettes sit in formalin for several weeks.

So I am assuming that the problem comes either in the processing or embedding stage.
I think the ink was still fine when I removed the cassette basket from the processor's retort, but I can't remember for sure.

Then, I let the cassettes sit in Surgipath Embedding Media for two hours before embedding the tissue, since my Shandon XP processor has only 2 wax reservoirs.
I have noticed lately that pieces of ink are coming off of the cassettes into the embedding media, making some-but not all-of the cassettes impossible to read..
I have turned down the temperature of the cassette holding tank, in case the temperature of the embedding media is pulling the ink off.

Has anyone experienced this before, and if so, how did you overcome it?
Are the Securline Marker 2/Superfrost pens appropriate for cassettes?

Thank you for any advice you can provide,

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA.

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