[Histonet] IHC H.P. Negative Control

[Sheila Fonner]

Rich,

 

That is definitely true also.  I guess I just assumed that the question was
in regards to 30 separate and individual patients.  My bad if that was not
the case.  Thanks for the clarification.

 

Sheila

 

 

From: Richard Yeo [mailto:ryeo <@t> wchosp.org] 
Sent: Thursday, September 29, 2011 12:16 PM
To: Sheila Fonner
Subject: RE: [Histonet] IHC H.P. Negative Control

 

Sheila,

 

Cap requires a negative with each block of patient tissue  being stained for
immunos, However they also state that if more than one block from the same
patient is being tested that only one of the blocks is required for the run.

 

 

Thanks Rich Y

 

  _____  

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Sheila Fonner
Sent: Thu 9/29/2011 11:31 AM
To: 'Adrienne Aperghis Kavanagh'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC H.P. Negative Control

CAP guidelines clearly state that you must run a negative control for each
patient tissue.  However, if you are running a large amount of a particular
antibody, you may validate your procedure to run only one positive control.
However, it is just as easy to run a positive control for each patient if
you put the control on the same slide as the patient tissue.

I don't see any way around the negatives though. We run a negative control
on each and every block.

Sheila


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Adrienne
Aperghis Kavanagh
Sent: Tuesday, September 27, 2011 1:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC H.P. Negative Control

Hi Histonetters,

We would like to start to run up to 30 H.Pylori on our Benchmark daily.  Do
GI labs that run H.P. on ALL stomach biopsies run a negative control for
each patient tissue for H.P.?  I am just curious, as we have a 24 hour TAT,
but the Benchmark only holds 30 slides.  What's everyone doing?

Thanks!

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