[Histonet] Azure B for melanin

[mesruh turkekul]

Dear Histology Gurus,

I read some where that one can do Azure B counter staining to stain the
melanin green to distinguish it from DAB in a IHC section that contains
brown melanin. Have anyone tried this? It seems less harmful to the
antigenicity of the tissue than bleaching with hydrogen peroxide or K
permanganate/oxalic acid treatment.


Any comments will be greatly appreciated!


Regards,
Mesruh Turkekul
mskcc.org