[Histonet] RE: Stain Precipitate (H.Pylori)

[Adrienne Aperghis Kavanagh]

We use H.Pylori from Cell Marque and the Benchmark XT as well.  When we began doing the H.Pylori by immuno (versus Giemsa) we had had the same problem.  Tech support from Cell Marque suggested using a Protease 2 (from Ventana) to decrese background, and it worked.  Here's our protocol:

CC1 Mild (30 min)
Protease 2 (4 min)
H.Pylori (32 min)
Hematox (4 min)
Bluing (4 min)

Hope this helps!!
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Adrienne Kavanagh  HTL (ASCP) 
US PATH 
30 W. Century Road 
Suite 255 
Paramus NJ 07652