[Histonet] Block age/labile epitopes - UPDATE

[Richard Cartun]

An interesting article on this subject came out earlier this year from
Stephen Hewitt's work group at the NCI.  Their work shows that the loss
of antigenicity in archival formalin-fixed, paraffin-embedded tissue
sections is due to the presence of water, both endogenously (poor
processing) and exogenously (humidity).  I've listed the reference
below:

Xie R, Chung J-Y, Ylaya K, et al.:  Factors influencing the degradation
of archival formalin-fixed, paraffin-embedded tissue sections.  J
Histochem Cytochem 59:356-365, 2011.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Rene J Buesa <rjbuesa <@t> yahoo.com> 9/25/2011 11:36 AM >>>
Andrea:
Under separate cover I am sending an article I wrote on the subject of
epitope oxidation including some  of theose easier to oxidize.
As to when a block is too old to be useless for IHC, that depends on
the epitope and the way the blocks have been stored.
I have been able to use 25 years old blocks and  the only thing you
have to do is to go deep into the block to reach an area where the
epitopes have not been oxidized yet.
René J.

--- On Sat, 9/24/11, Andrea T. Hooper <andreahooper <@t> rocketmail.com>
wrote:


From: Andrea T. Hooper <andreahooper <@t> rocketmail.com>
Subject: [Histonet] Block age/labile epitopes
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Saturday, September 24, 2011, 11:44 PM


Just curious as to at what shelf-life people consider their blocks too
"old" to rely on for IHC data? Along those lines I am wondering about
labile antigens ... does anyone know how fast epitopes may "disappear"
from blocks and what are some good examples of labile epitopes/antigens?
As usual any references or papers citations would be helpful.

Thank you!
Andrea Hooper
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