[Histonet] RE: Napsin A Controls
Meilus, Sheri D.
Sheri.Meilus <@t> va.gov
Wed Sep 21 12:38:09 CDT 2011
Normal lung also contains good internal positive controls such as Type II pneumocytes, histiocytes and macrophages.
Best regards,
S
Sheri Meilus
Anatomic Pathology Supervisor
Bay Pines VAHC
Building 100
Room 2B-126
727-398-6661 ext 4596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, September 21, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 94, Issue 24
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Today's Topics:
1. Legal Case SOP (Ann Angelo)
2. RE: Uni-trieve, antigen retrieval for immunofluorescence
(sdysart <@t> mirnarx.com)
3. cost of an H&E (Jennifer MacDonald)
4. RE: cost of an H&E (Burton, Lynn)
5. Effect of Freezing on Unfixed GFP (Andrew Coleman)
6. Re: Tissue handling at embedding (Rene J Buesa)
7. Re: cost of an H&E (Rene J Buesa)
8. Tissue handling at embedding (Tim Higgins)
9. RE: Uni-trieve, antigen retrieval for immunofluorescence
(Andrea Marion)
10. used microscope vendors (Caroline Bass)
11. C4d (Algeo, Lacie A)
12. FW: Procedure for SOS11 (Elizabeth.Dickert <@t> hcahealthcare.com)
13. unstructured proteins (Perry, Margaret)
14. RE: used microscope vendors (Rathborne, Toni)
15. Napsin A controls (Sara Baldwin/mhhcc.org)
16. Re: used microscope vendors (Rene J Buesa)
17. RE: Napsin A controls (Mike Pence)
18. Paid Survey ($50) Opportunity - Digital Pathology (Tieuvi Nguyen)
19. Klotz Solution (Breeden, Sara)
20. Urgent!! Need per diem (Heckford, Karen - SMMC-SF)
----------------------------------------------------------------------
Message: 1
Date: Tue, 20 Sep 2011 13:20:14 -0400 (EDT)
From: Ann Angelo <thisisann <@t> aol.com>
Subject: [Histonet] Legal Case SOP
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CE45D0B1BF937B-2198-21BC4 <@t> webmail-d008.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
Can anyone share their SOP regarding the handling of legal cases (I.e. if a specimen is being requested from an outside source which is not the patient). Thank you, Ann
------------------------------
Message: 2
Date: Tue, 20 Sep 2011 12:49:06 -0500
From: <sdysart <@t> mirnarx.com>
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
immunofluorescence
To: <amario3 <@t> uic.edu>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A2966B7 <@t> SVREXCH.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
I actually attended a class they put on a few months ago where they used
Unitrieve. It works really well. It doesn't seem to produce any kind
of background and yes, it is used at much lower temps. However the
retrieval process takes a little longer, but...you can put it in an even
lower temp. water bath overnight, come in to work in the morning, and
your slides are reading to go.
Contact Zara over there and she might be able to hook you up with a
sample.
Good Luck!!
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Marion
Sent: Tuesday, September 20, 2011 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
Hello all,
Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
to
be a universal antigen retrieval solution that can be used at lower
temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
antigens):
http://innvx.com/unitrievepage.html
The company claims that the reagent is a universal retrieval solution
for
all antibodies and tissues (which is silly of course - how could they
know?). Does anyone have any experience with the product?
I am interested because I see that increased heat during antigen
retrieval
causes greater tissue autofluorescence during immunofluorescence
stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
plate using sodium citrate buffer. Does anyone else either Uni-trieve or
a
different reagent/protocol for immunofluorescence stainings?
Thanks,
Andrea
Andrea Marion
Graduate Student
University of Illinois at Chicago
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 20 Sep 2011 11:23:55 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] cost of an H&E
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF844B0DFC.3746A884-ON88257911.0064EA4B-88257911.00651130 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
Has anyone calculated the approximate cost of an H&E, materials only, no
labor? Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
------------------------------
Message: 4
Date: Tue, 20 Sep 2011 13:30:12 -0500
From: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>
Subject: RE: [Histonet] cost of an H&E
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4A6E2CACA1E017408EBA1B9911952CC00643DD12DF <@t> IL084EXMBX214.illinois.gov>
Content-Type: text/plain; charset="us-ascii"
We calculated that to be right around $4.05 per slide. That does not include labor.
Lynn Burton
Lab Assoc I
Animal Disease Lab
Galesburg, Il
309-344-2451
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald [JMacDonald <@t> mtsac.edu]
Sent: Tuesday, September 20, 2011 1:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cost of an H&E
Has anyone calculated the approximate cost of an H&E, materials only, no
labor? Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Tue, 20 Sep 2011 15:50:51 -0400
From: Andrew Coleman <andrewcoleman131 <@t> gmail.com>
Subject: [Histonet] Effect of Freezing on Unfixed GFP
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAFAJL_zELXRwmCLXOG5RXHBfPMBKOXC5VO+-_ooVqvcWwMpERw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We have performed lentiviral injections with a turboGreenFluorescentProtein
(tGFP) into the brains of rats for an RNAi experiment. We did not fix the
tissue but froze in isopentane on dry ice and stored the tissue at -80 deg
Celsius.
We want to use some sections for a reference of the injection site (GFP
fluorescence) relative to the brain morphology (Neurotracer staining) in
order to selecitvely laser microdissect (LMD) infected cells from other
sections.
Our initial studies showed that fixation of sections on slides in 4%
paraformaledhyde (PFA) at 4 deg C for 5 minutes provided tGFP flourescence
comparable to that found in perfused tissue, but lately we see very weak or
no fluorescence with this procedure, even when increasing the time.
The only difference I can think of that we saw best results soon after the
brains were removed (ie 1 to 2 weeks) and now we are having trouble
visualizing the tGFP ~2 months of the brains being stored at -80 degrees
celsius.
Does anyone have experience with or an explanation of fluorescence
decreasing with a frozen, unfixed GFP over time?
In the future we plan on doing these experiments including a perfusion with
a low conc of PFA (to preserve RNA integrity) but for now we are trying to
figure out how to identify the GFP-positive cells in the animals we have
already performed surgery on. Another option would anti-tGFP IHC, but we
would prefer at this point to use on slide-fixed sections as a references
for our LMD.
Thanks! -Andrew
------------------------------
Message: 6
Date: Tue, 20 Sep 2011 12:51:45 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Tissue handling at embedding
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, TimothyMorken
<Timothy.Morken <@t> ucsfmedctr.org>
Message-ID:
<1316548305.7251.YahooMailClassic <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
This is how we do it:
1- the person grossing will dictate how many pieces should go into each cassette
2- the person cassetting will check that information against the vial with the pieces and write down in the cassettes summary form the number of pieces
3- the person embedding checks the pieces in the cassette after processing against the cassettes log.
If any piece is missing between any two steps, it will be looked for. Sometimes is small and is in the vial before cassetting, or small and in the retort.
Any irregularity is documented in the QA log.
René J.
--- On Tue, 9/20/11, Morken, Timothy <Timothy.Morken <@t> ucsfmedctr.org> wrote:
From: Morken, Timothy <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Tissue handling at embedding
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, September 20, 2011, 12:44 PM
What procedures do you have in place to prevent tissue loss at embedding? What do you do if tissue appears to have been lost?
And do you clean embedding molds before each reuse, or after one-days use (which may be many re-uses)?
Thanks for all info!
Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA
415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 20 Sep 2011 12:53:12 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] cost of an H&E
To: histonet <@t> lists.utsouthwestern.edu, Jennifer MacDonald
<JMacDonald <@t> mtsac.edu>
Message-ID:
<1316548392.93704.YahooMailClassic <@t> web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Under separate cover I am sending something I wrote about this subject.
René J.
--- On Tue, 9/20/11, Jennifer MacDonald <JMacDonald <@t> mtsac.edu> wrote:
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: [Histonet] cost of an H&E
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 2:23 PM
Has anyone calculated the approximate cost of an H&E, materials only, no
labor? Including the cost of the cassette, paraffin, slide, hematoxylin,
eosin and staining reagents?
Thanks,
Jennifer MacDonald
Mt. San Antonio College
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 20 Sep 2011 15:03:06 -0500
From: "Tim Higgins" <thiggins <@t> cddmedical.com>
Subject: [Histonet] Tissue handling at embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001101cc77d0$50b7afa0$e001a8c0 <@t> cdd.loc>
Content-Type: text/plain; charset="iso-8859-1"
I have worked at labs that reuse molds and ones that clean between uses.
Guess it all depends on what your definition of a "clean" mold is. If you
have the time and space, not reusing the molds is best practice but not
always practical.
Thanks,
Tim
Message: 10
Date: Tue, 20 Sep 2011 09:44:26 -0700
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Tissue handling at embedding
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8D7C2D242DBD45498006B21122072BF85E2AEE21 <@t> MCINFRWEM003.ucsfmedicalcenter.org
>
Content-Type: text/plain; charset=us-ascii
What procedures do you have in place to prevent tissue loss at embedding?
What do you do if tissue appears to have been lost?
And do you clean embedding molds before each reuse, or after one-days use
(which may be many re-uses)?
Thanks for all info!
Tim Morken
Supervisor, Histology, IPOX
UC San Francisco Medical Center
Box 1656
1600 Divisidero St, B217
San Francisco, CA 94115
USA
415.514.6042 (office)
415.885.7409 Fax
tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
------------------------------
Message: 9
Date: Tue, 20 Sep 2011 15:19:48 -0500
From: "Andrea Marion" <amario3 <@t> uic.edu>
Subject: RE: [Histonet] Uni-trieve, antigen retrieval for
immunofluorescence
To: sdysart <@t> mirnarx.com
Cc: histonet <@t> lists.utsouthwestern.edu, amario3 <@t> uic.edu
Message-ID:
<defdb03fcad8f889eb681e91977d8bee.squirrel <@t> webmail.uic.edu>
Content-Type: text/plain;charset=iso-8859-1
Hi Sarah,
Thanks for the info! Have you used Unitrieve for immunofluorescence? I am
wondering whether I will see a reduction in the autofluorescence caused by
heating? I will request a sample to try and report back to all.
Andrea
Andrea Marion
Graduate Student
University of Illinois at Chicago
On Tue, September 20, 2011 12:49 pm, sdysart <@t> mirnarx.com wrote:
> I actually attended a class they put on a few months ago where they used
> Unitrieve. It works really well. It doesn't seem to produce any kind
> of background and yes, it is used at much lower temps. However the
> retrieval process takes a little longer, but...you can put it in an even
> lower temp. water bath overnight, come in to work in the morning, and
> your slides are reading to go.
> Contact Zara over there and she might be able to hook you up with a
> sample.
> Good Luck!!
>
> Sarah Goebel-Dysart, BA, HT(ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
> Marion
> Sent: Tuesday, September 20, 2011 11:59 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence
>
> Hello all,
>
> Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported
> to
> be a universal antigen retrieval solution that can be used at lower
> temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear
> antigens):
>
> http://innvx.com/unitrievepage.html
>
> The company claims that the reagent is a universal retrieval solution
> for
> all antibodies and tissues (which is silly of course - how could they
> know?). Does anyone have any experience with the product?
>
> I am interested because I see that increased heat during antigen
> retrieval
> causes greater tissue autofluorescence during immunofluorescence
> stainings. My current protocol is to use 20 minutes at 90-95 C on a hot
> plate using sodium citrate buffer. Does anyone else either Uni-trieve or
> a
> different reagent/protocol for immunofluorescence stainings?
>
> Thanks,
>
> Andrea
>
> Andrea Marion
> Graduate Student
> University of Illinois at Chicago
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 10
Date: Tue, 20 Sep 2011 17:30:26 -0400
From: Caroline Bass <cebass <@t> buffalo.edu>
Subject: [Histonet] used microscope vendors
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49365679-58DD-48DE-80D5-0355BBA95515 <@t> buffalo.edu>
Content-Type: text/plain; charset=us-ascii
Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out.
Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.
Thanks,
Caroline
------------------------------
Message: 11
Date: Tue, 20 Sep 2011 16:21:05 -0700
From: "Algeo, Lacie A" <Lacie.Algeo <@t> providence.org>
Subject: [Histonet] C4d
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6A73DCDB9E46D2489D4001B4001C92AE012C4349 <@t> IRMEXCH05.irm.inhs.org>
Content-Type: text/plain; charset="us-ascii"
Hi Everyone,
Does anyone know of a FITC C4d that is ASR or IVD? All I have been able
to find is RUO. Also, is anyone using something different to show
rejection in transplant kidneys?
Thank you,
Lacie
Lacie Algeo, HTL, MP(ASCP)
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
Spokane, WA 99220
509-474-4418
FAX 509-474-2052
lacie.algeo <@t> providence.org
------------------------------
Message: 12
Date: Wed, 21 Sep 2011 05:45:14 -0500
From: <Elizabeth.Dickert <@t> hcahealthcare.com>
Subject: [Histonet] FW: Procedure for SOS11
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0226177269DFDE48A23F40D00634258B15EF6C83EE <@t> NADCWPMSGCMS10.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
_____________________________________________
From: Dickert Elizabeth
Sent: Thursday, September 15, 2011 10:35 AM
To: 'histonet-request <@t> lists.utsouthwestern.edu'
Subject: SOX11
Anyone doing a sox11 with good results?
Have Abcam antibody / Ventana equipment (ultraview detection)
Elizabeth Ann Dickert
Southern Pathology
2333 McCallie Ave.
Chattanooga, TN.
423-493-6904
elizabeth.dickert <@t> hcahealthcare.com
------------------------------
Message: 13
Date: Wed, 21 Sep 2011 12:55:47 +0000
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] unstructured proteins
To: "ihcrg <@t> googlegroups.com" <ihcrg <@t> googlegroups.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25F4FBA34BE9D142964ECC4525B82AEE012722 <@t> SDSU-EX03.jacks.local>
Content-Type: text/plain; charset="us-ascii"
Have any of you worked with the unstructured proteins? Do they form the same crosslinks when fixed with formalin? Would antigen retrieval be of any use?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
------------------------------
Message: 14
Date: Wed, 21 Sep 2011 13:54:00 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] used microscope vendors
To: "'Caroline Bass'" <cebass <@t> buffalo.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD061FE740D464FAC7BF6B5CFB7570711F56F73 <@t> SMCMAIL01.somerset-healthcare.com>
Content-Type: text/plain; charset="us-ascii"
What part of the country are you in?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Caroline Bass
Sent: Tuesday, September 20, 2011 5:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] used microscope vendors
Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out.
Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.
Thanks,
Caroline
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 15
Date: Wed, 21 Sep 2011 10:10:59 -0400
From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Subject: [Histonet] Napsin A controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OFB1EA30C6.094302F1-ON85257912.004DE8FD-85257912.004DE903 <@t> LocalDomain>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonetters
Are there any other controls for Napsin A other than Lung adenocarcinoma?
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-996-0210, 0216, Fax 812-996-0232,
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
------------------------------
Message: 16
Date: Wed, 21 Sep 2011 07:12:40 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] used microscope vendors
To: histonet <@t> lists.utsouthwestern.edu, Caroline Bass
<cebass <@t> buffalo.edu>
Message-ID:
<1316614360.28378.YahooMailClassic <@t> web65703.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Try eBay. I have seen good instruments for sale there.
René J.
--- On Tue, 9/20/11, Caroline Bass <cebass <@t> buffalo.edu> wrote:
From: Caroline Bass <cebass <@t> buffalo.edu>
Subject: [Histonet] used microscope vendors
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, September 20, 2011, 5:30 PM
Can anyone recommend a good source for either used microscopes or an inexpensive brand? I'm looking for a basic inverted microscope for very basic cell counts, and quick looks for cell viability and health. This is very much a quick in and out scope for students to kick around and abuse. I'm purchasing a fluorescent microscope for more extensive needs. I've looked on ebay and a couple other places, but the market is inundated with scopes that it makes it difficult to figure out.
Ideally, I'd love a recommendation for a basic used scope and maybe some names of reputable vendors of refurbished units.
Thanks,
Caroline
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Wed, 21 Sep 2011 09:15:32 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Napsin A controls
To: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C03974CBD <@t> is-e2k3.grhs.net>
Content-Type: text/plain; charset="US-ASCII"
Kidney
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Wednesday, September 21, 2011 9:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Napsin A controls
Hi Histonetters
Are there any other controls for Napsin A other than Lung
adenocarcinoma?
Thanks
Pathology Supervisor
S. Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-996-0210, 0216, Fax 812-996-0232,
Pager 812-481-0897, Cell 812-887-3357
Confidential information, Authorized use only.
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------------------------------
Message: 18
Date: Wed, 21 Sep 2011 08:22:15 -0700
From: Tieuvi Nguyen <tnguyen <@t> kaiserassociates.com>
Subject: [Histonet] Paid Survey ($50) Opportunity - Digital Pathology
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61C52EFB4F0AF8498D9ECADA6796B46620E26EDE <@t> VA3DIAXVS3D1.RED001.local>
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Hi Everyone,
I had sent a note out to the group about a month ago inviting participants to take part in phone interviews to discuss Digital Pathology technology. We are now following up those interviews with an online survey on the same subject. We are particularly interested in individuals that work in high volume commercial or reference labs (i.e. process more than 100,000 slides a year, and process more than 5,000 IHC slides a year). You do not have to be a current user of digital pathology to participate. The survey will begin with a few questions to determine if you qualify for the survey. If you qualify and complete the survey you will receive an honoraria in the form of a $50 American Express gift card for your participation. All answers will remain anonymous.
The survey should take about 15-20 minutes to complete.
You can access the survey here:
http://www.keysurvey.com/survey/387885/297f/
Please feel free to contact me if you have any questions.
Tieuvi Nguyen, PhD / Kaiser Associates, Inc.
M +1 202 506 0775 / tnguyen <@t> kaiserassociates.com
1201 Connecticut Ave NW, 12th Floor / Washington, DC 20036 USA
www.kaiserassociates.com
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Message: 19
Date: Wed, 21 Sep 2011 10:07:31 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Klotz Solution
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B051DFACC <@t> nmdamailsvr.nmda.ad.nmsu.edu>
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I've just "googled" and "histosearched" for Klotz solution but cannot
find anything that doesn't require chloral hydrate. Has anyone made a
Big Breakthrough in making Klotz that can be made without that pesky
controlled substance? Any help will be appreciated...who knew the
recipe joined the Witness Protection Program!
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 20
Date: Wed, 21 Sep 2011 09:29:07 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
Subject: [Histonet] Urgent!! Need per diem
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9ECF174E7DA83046BE6EBFDE009E28A388C456 <@t> CHW-MSG-301.chw.edu>
Content-Type: text/plain; charset="us-ascii"
I am looking for a Certified ASCP HistoTech. that is local to the San
Francisco Bay Area. I need this person to cover me while I am out
having knee surgery. I will be out 2-6 weeks probably more like 4 weeks
starting October 5th. But need somebody by October 3rd to do some
training.
You will need to know IHC and Special stains and be able to work
independently because I am the Chief, Cook and Bottle washer here.
Thanks,
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167Caution: This email message, including all
content and attachments, is CONFIDENTIAL and may be of a nature that is
LEGALLY PRIVILEGED. The information contained in this email message is
intended only for the use of the recipient(s) named above. If the reader
of this message is not the intended recipient or an agent responsible
for delivering it to the intended recipient, you have received this
document in error. Any further review, dissemination, distribution, or
copying of this message is strictly prohibited. If you have received
this communication in error, please notify us immediately by reply
email. Thank you."
------------------------------
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End of Histonet Digest, Vol 94, Issue 24
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