[Histonet] Spirochete Sensitizer

[Madill, Grant]

Hi Randi
I use 0.5% Iodine in 70% Alcohol as a pretreatment to the Warthin-Starry
method to enhance Leptospira, Brachyspira and Lawsonia sp.
I find it works very well.

After bringing sections to deionised water treat with 0.5% Iodine in 70%
Alcohol (prepare fresh) for 5 min. 
Treat in 5% Sodium Thiosulphate. The Iodine - brown (I2) immediately
becomes Iodide - colourless (I).
Wash in running tap water for 5 min. Rinse well in deionised water then
proceed with Warthin-Starry. 

I've found over the years that good silver staining depends on proper
fixation.

Hope this helps.
Grant Madill
Laboratory Technician 
Animal Disease Surveillance Laboratory
Biosecurity Queensland 
Department of Employment, Economic Development and Innovation
203 Tor Street, Toowoomba Qld 4350

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