[Histonet] immunohistochemistry: manual staining

[Paula Pierce]

Ditto the Sequenza!!!!

I work almost exclusively with eyes and they wash off the slides easily. The 
washes of the automated machines are too rough for them. The Sequenza is all I 
use!!!
 
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com




________________________________
From: John Shelley <jshelley <@t> sanfordburnham.org>
To: "wim.vandenbroeck <@t> UGent.be" <wim.vandenbroeck <@t> UGent.be>; 
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, September 13, 2011 8:05:45 AM
Subject: RE: [Histonet] immunohistochemistry: manual staining

I love to use the Thermo Shandon Sequenza system. You touch the slides one time 
as you place a cover-plate on them and then you just add your reagents at the 
top and let gravity and capillary effect do its job. If you would like more 
information just contact me directly. Thanks!!!

Kind Regards!
 
John J Shelley
Senior Research Associate, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim 
Van den Broeck
Sent: Tuesday, September 13, 2011 8:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining

Dear Colleagues,

We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ....) but I would like to make
things run more smoothly. So my question is, is there any device or systems
that can be helpful during manual IHC stainings ? 

Thanks in advance for your comments / suggestions.

-- 
Met vriendelijke groeten,


Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
Cell biology and Histology
Department of Morphology - Faculty of Veterinary Medicine
Ghent University
Salisburylaan 133, B-9820 Merelbeke, BELGIUM
wim.vandenbroeck <@t> UGent.be - tel.: +32 9 264 77 16 - fax: +32 9 264 77 90


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