[Histonet] RE: Leica Bond III

[Martha Ward]

We sometimes have issues with EBER and more often, with the Kappa and Lambda ISH on the Bond 3, more specifically on our tissues that were fixed in B Plus rather than formalin.  I would interested in the responses as well.

Martha Ward, MT (ASCP) QIHC
Manager, Molecular Diagnostics Lab
Dept. of Pathology
Wake Forest University Baptist Medical Center
Winston-Salem, NC 27157
336-716-2104



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Friday, September 02, 2011 10:51 AM
To: 'Diane.Craft <@t> amcny.org'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Bond III

I want to poll Leica Bond III users to see if they are able to successfully run EBER Insitu on their unit?
We continue to get inconsistent staining and inconsistent background staining.
I have been told it's a pH problem, it's a tissue processing problem but we are able to do the stain with great results by hand. 
I welcome any and all suggestions  


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