[Histonet] Contract to Hire Opportunity in SF Bay Area.
Erik Dokken
Erik.Dokken <@t> onassignment.com
Mon Oct 31 15:11:05 CDT 2011
We have an immediate full time opening for a qualified Histo Tech in the SF Bay Area.
If you would like additional information please contact me directly.
Erik Dokken
Area Manager - No. CA, WA, and TX
On Assignment, Inc. Local Allied Healthcare
fax. 866-604-0193
NASDAQ: ASGN
www.oahealthcare.com
People First
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, October 31, 2011 9:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 95, Issue 37
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Today's Topics:
1. For zebrafish larvae section (=?gb2312?B?tNTk7LfJ?=)
2. Re: Stripping antibodies (Claire Weston)
3. Re: Stripping antibodies (John Kiernan)
4. RE: SALARY (Heath, Nancy L.)
5. RE: SALARY (Heath, Nancy L.)
6. RE: SALARY (Heath, Nancy L.)
7. Must be Monday... (Breeden, Sara)
8. RE: Must be Monday... (Blazek, Linda)
9. Florida Medical Directorship (Hale, Meredith)
10. Problems with Frozen tissues (Igor Deyneko)
11. Stripping antibodies (C.M. van der Loos)
12. RE: Must be Monday... (Beckham, Sharon)
13. RE: SALARY (Mayer,Toysha N)
14. RE: SALARY (joelle weaver)
15. RE: need help with TMA (Helen Fedor)
16. RE: RE: SALARY (joelle weaver)
17. background checks required by AHCA (Nicole Tatum)
18. Dako Pr on Ventana? (Orr, Rebecca)
----------------------------------------------------------------------
Message: 1
Date: Mon, 31 Oct 2011 02:21:03 +0800
From: =?gb2312?B?tNTk7LfJ?= <congxf20002 <@t> hotmail.com>
Subject: [Histonet] For zebrafish larvae section
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT131-W5248EDA6E3DBF33E9D1658E4D10 <@t> phx.gbl>
Content-Type: text/plain; charset="gb2312"
Hi,
My respects! I wanted to do some zebrafish larvae sectioning. But some slice I made showed that the tissue inside had been broken. As to me, I thought the problem might be in dehydration time and and the razor I used. So can you please give me some advice to improve the process? In addition, I wanted to make some cross section. But it was difficult to me to control the orientation of the larvae. Thanks a lot!
Best,Joseph Cong
------------------------------
Message: 2
Date: Sun, 30 Oct 2011 11:58:19 -0700
From: "Claire Weston" <cweston <@t> valasciences.com>
Subject: Re: [Histonet] Stripping antibodies
To: "John Kiernan" <jkiernan <@t> uwo.ca>
Cc: histonet <@t> lists.utsouthwestern.edu, amosbrooks <@t> gmail.com
Message-ID: <20111030115819.1ECE91E5 <@t> resin12.mta.everyone.net>
Content-Type: text/plain; charset="UTF-8"
<DIV style="font-family:Arial, sans-serif; font-size:10pt;"><DIV>Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best.</DIV>
<DIV> </DIV>
<DIV>Thanks again for all your help,</DIV>
<DIV> </DIV>
<DIV>Claire <BR><BR>--- jkiernan <@t> uwo.ca wrote:<BR><BR>From: John Kiernan <jkiernan <@t> uwo.ca><BR>To: histonet <@t> lists.utsouthwestern.edu, cweston <@t> valasciences.com<BR>Cc: Amos Brooks <amosbrooks <@t> gmail.com><BR>Subject: Re: [Histonet] Stripping antibodies<BR>Date: Sun, 30 Oct 2011 12:09:58 -0400<BR><BR></DIV>
<DIV><SPAN>
<DIV>Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.</DIV>
<DIV> </DIV>
<DIV>Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.</DIV>
<DIV> </DIV>
<DIV>Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good.</DIV>
<DIV> </DIV>
<DIV>John Kiernan</DIV>
<DIV>Anatomy, UWO </DIV>
<DIV>London, Canada </DIV>
<DIV>= = = =</DIV>On 29/10/11, <B>Amos Brooks </B><amosbrooks <@t> gmail.com> wrote:</SPAN>
<BLOCKQUOTE style="BORDER-LEFT: #00f 1px solid; PADDING-LEFT: 13px; MARGIN-LEFT: 0px">
<DIV>Hi,<BR> Stripping sites are usually found in the more seedy areas of large<BR>cities ... Oh wait you meant ... nevermind :-) If you developed the reaction<BR>with DAB, you may be in a difficult position. DAB is a really strong<BR>reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in<BR>5% potassium premanganate for 5 min followed by 5% oxalic acid until it is<BR>clear (usually a couple of minutes). Unfortunately, I am not sure what<BR>effect this will have on the epitopes you are looking for, but this is<BR>usually what is used to clean precipitated DAB from instruments it should<BR>work on the tissue too.<BR> If you use another chromogen such as AEC it is much easier (to many<BR>people's dismay) to remove this end product. Using alkaline phosphatase will<BR>be easy to remove the chromogens as well. It would also be very easy to do<BR>this with fluorescent tags too. Just remove the coverslip and dip the slide<BR>in ethanol and it will remove the colored end product. Then just start over<BR>for the new antigen.<BR><BR>Good luck,<BR>Amos<BR><BR>On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request <@t> lists.utsouthwestern.edu<BR>> wrote:<BR><BR>> Message: 2<BR>> Date: Thu, 27 Oct 2011 10:30:40 -0700<BR>> From: "Claire Weston" <cweston <@t> valasciences.com><BR>> Subject: [Histonet] Stripping antibodies<BR>> To: <histonet <@t> lists.utsouthwestern.edu><BR>> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com><BR>> Content-Type: text/plain; charset="us-ascii"<BR>><BR>> Hi!<BR>><BR>><BR>><BR>> Does anyone have experience in IHC stripping antibodies from a tissue<BR>> section and re-probing with different antibodies? What is the best way to<BR>> do that? If you could share your stripping protocol or experience I would<BR>> appreciate it - thanks!<BR>><BR>><BR>><BR>> Claire<BR>><BR>_______________________________________________<BR>Histonet mailing list<BR>Histonet <@t> lists.utsouthwestern.edu<BR><A href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR></DIV></BLOCKQUOTE>
<DIV> </DIV> </DIV>
<DIV> </DIV><SPAN>On 29/10/11, <B>Amos Brooks </B><amosbrooks <@t> gmail.com> wrote:</SPAN>
<BLOCKQUOTE style="BORDER-LEFT: #00f 1px solid; PADDING-LEFT: 13px; MARGIN-LEFT: 0px">
<DIV>Hi,<BR> Stripping sites are usually found in the more seedy areas of large<BR>cities ... Oh wait you meant ... nevermind :-) If you developed the reaction<BR>with DAB, you may be in a difficult position. DAB is a really strong<BR>reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in<BR>5% potassium premanganate for 5 min followed by 5% oxalic acid until it is<BR>clear (usually a couple of minutes). Unfortunately, I am not sure what<BR>effect this will have on the epitopes you are looking for, but this is<BR>usually what is used to clean precipitated DAB from instruments it should<BR>work on the tissue too.<BR> If you use another chromogen such as AEC it is much easier (to many<BR>people's dismay) to remove this end product. Using alkaline phosphatase will<BR>be easy to remove the chromogens as well. It would also be very easy to do<BR>this with fluorescent tags too. Just remove the coverslip and dip the slide<BR>in ethanol and it will remove the colored end product. Then just start over<BR>for the new antigen.<BR><BR>Good luck,<BR>Amos<BR><BR>On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request <@t> lists.utsouthwestern.edu<BR>> wrote:<BR><BR>> Message: 2<BR>> Date: Thu, 27 Oct 2011 10:30:40 -0700<BR>> From: "Claire Weston" <cweston <@t> valasciences.com><BR>> Subject: [Histonet] Stripping antibodies<BR>> To: <histonet <@t> lists.utsouthwestern.edu><BR>> Message-ID: <002401cc94ce$2619ca60$724d5f20$@com><BR>> Content-Type: text/plain; charset="us-ascii"<BR>><BR>> Hi!<BR>><BR>><BR>><BR>> Does anyone have experience in IHC stripping antibodies from a tissue<BR>> section and re-probing with different antibodies? What is the best way to<BR>> do that? If you could share your stripping protocol or experience I would<BR>> appreciate it - thanks!<BR>><BR>><BR>><BR>> Claire<BR>><BR>_______________________________________________<BR>Histonet mailing list<BR>Histonet <@t> lists.utsouthwestern.edu<BR><A href="http://lists.utsouthwestern.edu/mailman/listinfo/histonet">http://lists.utsouthwestern.edu/mailman/listinfo/histonet</A><BR></DIV></BLOCKQUOTE>
<DIV> </DIV></DIV>
------------------------------
Message: 3
Date: Mon, 31 Oct 2011 02:12:52 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Stripping antibodies
To: cweston <@t> valasciences.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <7690e6ad626d2.4eae0424 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII
With quantum dots and a modern fluorescence microscope with "channels" it should be possible to label 2 or 3 antigens simultaneously, if you have all the right primaries and labelled secondaries. The company that sells you the quantum dot labelled secondaries or other amplification reagents should be in a position to tell you exactly what to do. Do they refund your hard-earned grant money if their expensive product fails to perform as advertized?
Immunohistochemistry with non-fluorescent colours (available in brown, black, red and various blues) has advantages:
(a) You do not need a fluorescence microscope. Modern fluorescence microscopes are very expensive; the old ones from the 1960s are no longer be good enough to provide publication-quality pictures.
(b) Immunostained slides can be kept for many years in boxes at room temperature.
(c) The colours do not fade in permanently mounted preparations.
(c) You are not bound to use a commercially sold kit, which may not be optimized for your research.
If you go by the book (= any book with references that you can follow up), you will do your multiple immunostains intelligently. Never follow a set of instructions without knowing or questioning the reason for ever step.
John Kiernan
Anatomy, UWO
London, Canada.
= = =
On 30/10/11, Claire Weston <cweston <@t> valasciences.com> wrote:
>
>
>
> Thank you everyone for your advice, I really appreciate it! I am doing a multiplex immunofluorescent assay (4 channels) and I am planning to strip the antibodies and reprobe with a second round of antibodies. The antibodies are labelled with quantum dots so I am not sure how that will influence things, but luckily I won't have the DAB problem several of you mentioned. I think I will try several of these techniques and evaluate which works the best.
>
> Thanks again for all your help,
>
> Claire
>
> --- jkiernan <@t> uwo.ca wrote:
>
> From: John Kiernan <jkiernan <@t> uwo.ca>
> To: histonet <@t> lists.utsouthwestern.edu, cweston <@t> valasciences.com
> Cc: Amos Brooks <amosbrooks <@t> gmail.com>
> Subject: Re: [Histonet] Stripping antibodies
> Date: Sun, 30 Oct 2011 12:09:58 -0400
>
>
>
> Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.
>
> Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.
>
> Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good.
>
> John Kiernan
> Anatomy, UWO
> London, Canada
> = = = =
> On 29/10/11, Amos Brooks <amosbrooks <@t> gmail.com> wrote:
>
> >
> > Hi,
> > Stripping sites are usually found in the more seedy areas of large
> > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
> > with DAB, you may be in a difficult position. DAB is a really strong
> > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
> > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
> > clear (usually a couple of minutes). Unfortunately, I am not sure what
> > effect this will have on the epitopes you are looking for, but this is
> > usually what is used to clean precipitated DAB from instruments it should
> > work on the tissue too.
> > If you use another chromogen such as AEC it is much easier (to many
> > people's dismay) to remove this end product. Using alkaline phosphatase will
> > be easy to remove the chromogens as well. It would also be very easy to do
> > this with fluorescent tags too. Just remove the coverslip and dip the slide
> > in ethanol and it will remove the colored end product. Then just start over
> > for the new antigen.
> >
> > Good luck,
> > Amos
> >
> > On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request <@t> lists.utsouthwestern.edu
> > > wrote:
> >
> > > Message: 2
> > > Date: Thu, 27 Oct 2011 10:30:40 -0700
> > > From: "Claire Weston" <cweston <@t> valasciences.com>
> > > Subject: [Histonet] Stripping antibodies
> > > To: <histonet <@t> lists.utsouthwestern.edu>
> > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> > > Content-Type: text/plain; charset="us-ascii"
> > >
> > > Hi!
> > >
> > >
> > >
> > > Does anyone have experience in IHC stripping antibodies from a tissue
> > > section and re-probing with different antibodies? What is the best way to
> > > do that? If you could share your stripping protocol or experience I would
> > > appreciate it - thanks!
> > >
> > >
> > >
> > > Claire
> > >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
>
>
>
> On 29/10/11, Amos Brooks <amosbrooks <@t> gmail.com> wrote:
>
> >
> > Hi,
> > Stripping sites are usually found in the more seedy areas of large
> > cities ... Oh wait you meant ... nevermind :-) If you developed the reaction
> > with DAB, you may be in a difficult position. DAB is a really strong
> > reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in
> > 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is
> > clear (usually a couple of minutes). Unfortunately, I am not sure what
> > effect this will have on the epitopes you are looking for, but this is
> > usually what is used to clean precipitated DAB from instruments it should
> > work on the tissue too.
> > If you use another chromogen such as AEC it is much easier (to many
> > people's dismay) to remove this end product. Using alkaline phosphatase will
> > be easy to remove the chromogens as well. It would also be very easy to do
> > this with fluorescent tags too. Just remove the coverslip and dip the slide
> > in ethanol and it will remove the colored end product. Then just start over
> > for the new antigen.
> >
> > Good luck,
> > Amos
> >
> > On Fri, Oct 28, 2011 at 11:50 AM, <histonet-request <@t> lists.utsouthwestern.edu
> > > wrote:
> >
> > > Message: 2
> > > Date: Thu, 27 Oct 2011 10:30:40 -0700
> > > From: "Claire Weston" <cweston <@t> valasciences.com>
> > > Subject: [Histonet] Stripping antibodies
> > > To: <histonet <@t> lists.utsouthwestern.edu>
> > > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com>
> > > Content-Type: text/plain; charset="us-ascii"
> > >
> > > Hi!
> > >
> > >
> > >
> > > Does anyone have experience in IHC stripping antibodies from a tissue
> > > section and re-probing with different antibodies? What is the best way to
> > > do that? If you could share your stripping protocol or experience I would
> > > appreciate it - thanks!
> > >
> > >
> > >
> > > Claire
> > >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
>
>
>
>
>
------------------------------
Message: 4
Date: Mon, 31 Oct 2011 06:20:57 -0400
From: "Heath, Nancy L." <NHeath <@t> Lifespan.org>
Subject: RE: [Histonet] SALARY
To: "Richard Cartun" <Rcartun <@t> harthosp.org>, <kcastillo <@t> frii.com>,
<Histonet <@t> lists.utsouthwestern.edu>, "Caroline Pratt"
<Caroline.Pratt <@t> uphs.upenn.edu>
Message-ID:
<130E8991F210424096EFC6F42EA33B240843564F <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
"Histotechnologists are the most valuable employees in the laboratory
today!"
THANK YOU DR. CARTUN!!
Nancy Heath, HT (ASCP)
Neuropathology Technician
Neuropathology Department
Rhode Island Hospital
President - Rhode Island Society for Histotechnology
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Friday, October 28, 2011 12:52 PM
To: kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu; Caroline
Pratt
Subject: RE: [Histonet] SALARY
I think that's low. If you find a good candidate with years of
experience I would pay them whatever it takes to get them in the door.
It's like "Free Agency" in baseball; if you want a good player, you need
to put the "money on table". Histotechnologists are the most valuable
employees in the laboratory today!
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu> 10/28/2011 9:21 AM
>>>
I would say $28 to $32 dollars an hour depending on experience and
education.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
kcastillo <@t> frii.com
Sent: Friday, October 28, 2011 7:54 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] SALARY
HI EVERYONE,
WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID
THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM
LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information contained in this e-mail message is intended only for
the personal and confidential use of the recipient(s) named above. If
the reader of this message is not the intended recipient or an agent
responsible for delivering it to the intended recipient, you are hereby
notified that you have received this document in error and that any
review, dissemination, distribution, or copying of this message is
strictly prohibited. If you have received this communication in error,
please notify us immediately by e-mail, and delete the original message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 31 Oct 2011 06:29:12 -0400
From: "Heath, Nancy L." <NHeath <@t> Lifespan.org>
Subject: RE: [Histonet] SALARY
To: "John Shelley" <jshelley <@t> sanfordburnham.org>, "Pratt, Caroline"
<Caroline.Pratt <@t> uphs.upenn.edu>, "Richard Cartun"
<Rcartun <@t> harthosp.org>, <kcastillo <@t> frii.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<130E8991F210424096EFC6F42EA33B2408435651 <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket.
I'm in New England.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Shelley
Sent: Friday, October 28, 2011 2:25 PM
To: Pratt, Caroline; Richard Cartun; kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] SALARY
With all that said what are your going rates in this area that you are speaking.
Kind Regards!
?
John J Shelley
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
Sent: Friday, October 28, 2011 2:19 PM
To: Richard Cartun; kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] SALARY
If that is an option, I would agree, but non-profit teaching hospitals
have compensation ranges and grids according to education and
experience. We keep every employee with the same education and skill
set at the same compensation for an equitable pay system. If we feel
our employees are being under paid we will conduct a market analysis and
the rates will be increased for all applicable employees if the market
analysis justifies it.
Car :)
-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
Sent: Friday, October 28, 2011 12:52 PM
To: kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu; Pratt,
Caroline
Subject: RE: [Histonet] SALARY
I think that's low. If you find a good candidate with years of
experience I would pay them whatever it takes to get them in the door.
It's like "Free Agency" in baseball; if you want a good player, you need
to put the "money on table". Histotechnologists are the most valuable
employees in the laboratory today!
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu> 10/28/2011 9:21 AM
>>>
I would say $28 to $32 dollars an hour depending on experience and
education.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
kcastillo <@t> frii.com
Sent: Friday, October 28, 2011 7:54 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] SALARY
HI EVERYONE,
WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID
THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM
LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information contained in this e-mail message is intended only for
the personal and confidential use of the recipient(s) named above. If
the reader of this message is not the intended recipient or an agent
responsible for delivering it to the intended recipient, you are hereby
notified that you have received this document in error and that any
review, dissemination, distribution, or copying of this message is
strictly prohibited. If you have received this communication in error,
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------------------------------
Message: 6
Date: Mon, 31 Oct 2011 06:30:04 -0400
From: "Heath, Nancy L." <NHeath <@t> Lifespan.org>
Subject: RE: [Histonet] SALARY
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>, "Podawiltz, Thomas"
<tpodawiltz <@t> lrgh.org>, "Richard Cartun" <Rcartun <@t> harthosp.org>,
<kcastillo <@t> frii.com>, <Histonet <@t> lists.utsouthwestern.edu>, "Caroline
Pratt" <Caroline.Pratt <@t> uphs.upenn.edu>
Message-ID:
<130E8991F210424096EFC6F42EA33B2408435652 <@t> LSCOEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
I agree :)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Friday, October 28, 2011 3:22 PM
To: Podawiltz, Thomas; Richard Cartun; kcastillo <@t> frii.com;
Histonet <@t> lists.utsouthwestern.edu; Caroline Pratt
Subject: RE: [Histonet] SALARY
I nominate Dr. Cartun as the Patron Saint for Histopathology. We should
work on a statue and a Mission Statement for him...
_______________________________________________
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------------------------------
Message: 7
Date: Mon, 31 Oct 2011 06:33:01 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Must be Monday...
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B051DFB81 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Here I sit, languishing as if I had nothing at all to do. And why, you
ask, is that? It seems that Daylight Savings Time of Olde has taken
command of my processor. Nothing on any calendar I've got here in the
lab says anything about DST being a factor for this weekend - and, in
fact, my calendar says NEXT Sunday is DST. So I have time to agitate
Histonet, having about 45 minutes left to entertain myself. And it is
Halloween...
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 8
Date: Mon, 31 Oct 2011 08:40:02 -0400
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: [Histonet] RE: Must be Monday...
To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5A2BD13465E061429D6455C8D6B40E39127401B441 <@t> IBMB7Exchange.digestivespecialists.com>
Content-Type: text/plain; charset="us-ascii"
That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date.
Linda
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, October 31, 2011 8:33 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Must be Monday...
Here I sit, languishing as if I had nothing at all to do. And why, you
ask, is that? It seems that Daylight Savings Time of Olde has taken
command of my processor. Nothing on any calendar I've got here in the
lab says anything about DST being a factor for this weekend - and, in
fact, my calendar says NEXT Sunday is DST. So I have time to agitate
Histonet, having about 45 minutes left to entertain myself. And it is
Halloween...
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
_______________________________________________
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------------------------------
Message: 9
Date: Mon, 31 Oct 2011 08:01:31 -0500
From: "Hale, Meredith" <mhale <@t> carisls.com>
Subject: [Histonet] Florida Medical Directorship
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6F33D8418806044682A391273399860F0A5B2974 <@t> s-irv-ex301.PathologyPartners.intranet>
Content-Type: text/plain; charset="us-ascii"
What are the true regulations' in Florida as far as time required for a
Medical Director to be on site at the lab ? I am finding conflicting
information and would like to know what is truly required. Thanks
Meredith Hale HT (ASCP)cm
Operations Liaision Director and Education Coordinator
Caris Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
------------------------------
Message: 10
Date: Mon, 31 Oct 2011 09:56:10 -0400
From: Igor Deyneko <igor.deyneko <@t> gmail.com>
Subject: [Histonet] Problems with Frozen tissues
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CA+48pcCV3iThmSUbXOG=BAqUJETxwKjRtVhxAM0N__GCqDkgZQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I'm looking for some advice on frozen tissues. This is the first time I'm
doing it. All the tissues: skin, lungs, spleen, liver, and pancreas cut
well onto special Gold Plus slides from Fisher. Then, when I was ready to
stain the slides, i air dried them fro an hour and wanted to do H&E and
Beta-Gal, all the tissues fell off slides. Can anyone suggest any tips on
preventing this mischief?
Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA
------------------------------
Message: 11
Date: Mon, 31 Oct 2011 14:02:04 +0000
From: "C.M. van der Loos" <c.m.vanderloos <@t> amc.uva.nl>
Subject: [Histonet] Stripping antibodies
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "'amosbrooks <@t> gmail.com'" <amosbrooks <@t> gmail.com>
Message-ID:
<BDA3BC4760AFA841926D5FCE9D0C16801B110B <@t> MS-APP-804C.amc.intra>
Content-Type: text/plain; charset="us-ascii"
Amos and John,
I do not agree with John saying that "antibodies could be easily removed by acidity pH1-2". High affinity primaries will stay at the tissue section nicely bound to the epitope. Try for example SMA, clone 1A4 antibody in the usual dilution. After this incubation strip off the antibody by glycin-HCl buffer pH2 and then apply an anti-mouse detection system. You will stain SMA for sure. The only way to get antibodies off is 10 min boiling with citrate pH6.0 or so, as in HIER. I have described that in JOH in 2008, vol 31, pp. 119-127, being the basis of an AP sequential double staining.
Chris van der Loos
Academic Medical Center
Dept. of Pathology M2-230
Amsterdam
The Netherlands
Date: Sun, 30 Oct 2011 12:09:58 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Stripping antibodies
To: histonet <@t> lists.utsouthwestern.edu, cweston <@t> valasciences.com
Cc: Amos Brooks <amosbrooks <@t> gmail.com>
Message-ID: <7640acec48487.4ead3e96 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII
Antibodies are quite easily removed by acidity - pH 1 to 2. This also removes enzyme-antibody conjugates. The coloured products of the enzyme histochemical reactions are generally not affected. The brown oxidation product of DAB (from peroxidase) and the blue products from bromochloroindoxyl/tetrazolium methods (for alkaline phosphatase) are very stable. This is good: you can remove all the primary and enzyme-labelled antibodies without losing track of the stained antigen in the section. A second immunostaining, with a differently coloured end-product can follow. If you use AEC as a peroxidase chromogen (red), it should be for the last immunohistochemical procedure, and you will have to use an aqueous mounting medium. The AEC oxidation product dissolves in alcohol and other organic solvents.
Amos suggests a strong oxidation (acid permanganate followed by oxalic acid to remove brown manganese dioxide) to get rid of the insoluble brown oxidation product of DAB. Is this what you want? This oxidation converts the sulphur-containing amino acids, including cystine -S-S- links, to sulphonic acids, which attract cationic dyes even at pH 1. This oxidation couuld seriously modify the epitopes for the next applied primary antibody.
Multicolour immunohistochemistry has been a developed technology for several years. Any lab needing to do such work needs to take training courses (hundreds of dollars) or buy a book (tens of dollars). The one by Chris van der Loos, Immunoenzyme Multiple Staining Methods, Oxford, UK: Bios, 1999, is very good.
John Kiernan
Anatomy, UWO
London, Canada
------------------------------
Message: 12
Date: Mon, 31 Oct 2011 09:22:38 -0500
From: "Beckham, Sharon" <SLB <@t> stowers.org>
Subject: [Histonet] RE: Must be Monday...
To: "'Blazek, Linda'" <lblazek <@t> digestivespecialists.com>, "'Breeden,
Sara'" <sbreeden <@t> nmda.nmsu.edu>, "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C40E43D1F7A56408C4463FD245DDDF98AEE2C42 <@t> EXCHMB-02.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Yes it was this past weekend back when. My alarm clock went to DST Sunday morning.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Monday, October 31, 2011 7:40 AM
To: 'Breeden, Sara'; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Must be Monday...
That's a hoot Sally! I don't think that DST of old was even this past weekend. For the past 3 years my processor changed on the DST of old and the powers that be told me it was impossible because that wasn't programmed into my processor's data. This year I forgot about it and it didn't change on the old date.
Linda
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Monday, October 31, 2011 8:33 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Must be Monday...
Here I sit, languishing as if I had nothing at all to do. And why, you ask, is that? It seems that Daylight Savings Time of Olde has taken command of my processor. Nothing on any calendar I've got here in the lab says anything about DST being a factor for this weekend - and, in fact, my calendar says NEXT Sunday is DST. So I have time to agitate Histonet, having about 45 minutes left to entertain myself. And it is Halloween...
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 31 Oct 2011 09:25:58 -0500
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE: SALARY
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DFD2C49464F83A4F9201E2D07100774E1BAEB983D8 <@t> DCPWVMBXC0VS3.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility.
In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area.
Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more.
My first supervisor job paid $32, and I worked like crazy. It wasn't worth it.
You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org
Message: 6
Date: Fri, 28 Oct 2011 12:52:23 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: RE: [Histonet] SALARY
To: <kcastillo <@t> frii.com>,<Histonet <@t> lists.utsouthwestern.edu>,
"Caroline Pratt" <Caroline.Pratt <@t> uphs.upenn.edu>
Message-ID: <4EAAA586.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today!
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu> 10/28/2011 9:21 AM >>>
I would say $28 to $32 dollars an hour depending on experience and education.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kcastillo <@t> frii.com
Sent: Friday, October 28, 2011 7:54 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] SALARY
HI EVERYONE,
WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID
THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM
LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY
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The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.
------------------------------
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End of Histonet Digest, Vol 95, Issue 34
****************************************
------------------------------
Message: 14
Date: Mon, 31 Oct 2011 14:35:21 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] SALARY
To: <nheath <@t> lifespan.org>, <jshelley <@t> sanfordburnham.org>,
<caroline.pratt <@t> uphs.upenn.edu>, <rcartun <@t> harthosp.org>,
<kcastillo <@t> frii.com>, Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-W388BC2D7D02D2236267A95D8D60 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I know the cost of living is greater on the east coast, but that sounds good to me. The insurance is not better, and as an HTL I make less than 1/2 of that! Things are determined by the market I realize, but all these postings are sure making me feel bad and also realize that I am not marketing myself well....
Joelle Weaver MAOM, BA, (HTL) ASCP
> Date: Mon, 31 Oct 2011 06:29:12 -0400
> From: NHeath <@t> Lifespan.org
> To: jshelley <@t> sanfordburnham.org; Caroline.Pratt <@t> uphs.upenn.edu; Rcartun <@t> harthosp.org; kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] SALARY
> CC:
>
> Just got offered a lead tech position, salary of $85600.00 in a private lab opened by a group of docs. Didn't take it because the health insurance plan they offered was lousey. 75% health covered with a 250.00 deductable, dental covered at 60% and no coverage for family or husband....would of had to pay that out of pocket.
> I'm in New England.
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Shelley
> Sent: Friday, October 28, 2011 2:25 PM
> To: Pratt, Caroline; Richard Cartun; kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] SALARY
>
> With all that said what are your going rates in this area that you are speaking.
>
> Kind Regards!
>
> John J Shelley
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pratt, Caroline
> Sent: Friday, October 28, 2011 2:19 PM
> To: Richard Cartun; kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] SALARY
>
> If that is an option, I would agree, but non-profit teaching hospitals
> have compensation ranges and grids according to education and
> experience. We keep every employee with the same education and skill
> set at the same compensation for an equitable pay system. If we feel
> our employees are being under paid we will conduct a market analysis and
> the rates will be increased for all applicable employees if the market
> analysis justifies it.
>
> Car :)
>
> -----Original Message-----
> From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
> Sent: Friday, October 28, 2011 12:52 PM
> To: kcastillo <@t> frii.com; Histonet <@t> lists.utsouthwestern.edu; Pratt,
> Caroline
> Subject: RE: [Histonet] SALARY
>
> I think that's low. If you find a good candidate with years of
> experience I would pay them whatever it takes to get them in the door.
> It's like "Free Agency" in baseball; if you want a good player, you need
> to put the "money on table". Histotechnologists are the most valuable
> employees in the laboratory today!
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
> >>> "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu> 10/28/2011 9:21 AM
> >>>
> I would say $28 to $32 dollars an hour depending on experience and
> education.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> kcastillo <@t> frii.com
> Sent: Friday, October 28, 2011 7:54 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] SALARY
>
> HI EVERYONE,
>
> WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID
> THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM
> LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information contained in this e-mail message is intended only for
> the personal and confidential use of the recipient(s) named above. If
> the reader of this message is not the intended recipient or an agent
> responsible for delivering it to the intended recipient, you are hereby
> notified that you have received this document in error and that any
> review, dissemination, distribution, or copying of this message is
> strictly prohibited. If you have received this communication in error,
> please notify us immediately by e-mail, and delete the original message.
>
>
>
>
>
> ------------------------------------------------------------------------
> -
> This message was secured by ZixCorp(R).
>
>
> The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message.
> _______________________________________________
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>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 15
Date: Mon, 31 Oct 2011 14:36:26 +0000
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: [Histonet] RE: need help with TMA
To: 'Patricia F Lott' <plott <@t> uab.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BCE07FB3A86FD040969A9EC0DD3EDA0D0B87D532 <@t> JHEMTMWEX4.win.ad.jhu.edu>
Content-Type: text/plain; charset="us-ascii"
Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply gentle pressure to center of block and slide complex, cool to RT and repeat 1 or two times.
Helen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patricia F Lott
Sent: Friday, October 28, 2011 1:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] need help with TMA
We have recently started doing tissue microarrays. The problem we have is that some of the small cores drop out after the first few sections.
I think my problem is in the step where we are supposed to melt the donor and recipient blocks together. We tried 5 minutes at 37 degrees, and then minutes at 60 degrees x3, with cooling to room temp in between.
Any suggestions?
Thanks,
Patty Lott, UAB
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------------------------------
Message: 16
Date: Mon, 31 Oct 2011 14:40:13 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] RE: SALARY
To: <tnmayer <@t> mdanderson.org>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT135-W24DF72979F79E999A53359D8D60 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Still sounds good to me, at my first supervisor's job ( the credentials I had at the time were HTL + Bachelor's), and I made less than $23 per hour and worked an average of 16 hours a day with no OT or comp time that was ever granted ( though promised). I guess I am just trying to say, count your blessings. Having a school near by really seems to impact the supply and demand and the general hiring environment. What I have noticed also is that when you go somewhere and the cost of living is less the tax burden is greater, so it has always seemed to come "out in the wash". Anyhow, all these salary revelations have really made me think, thanks for the postings.
Joelle Weaver MAOM, BA, (HTL) ASCP
> From: TNMayer <@t> mdanderson.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 31 Oct 2011 09:25:58 -0500
> Subject: [Histonet] RE: SALARY
>
> Add location to that. $28-32 is great for places that do not have an abundant supply of techs. Here in Houston, we have 2 schools and a very large medical center. Entry level techs are not hard to find. Graduates expect $23-28 minimum depending on facility.
> In other states, neighboring Louisiana and such, they expect $21-24. It just depends on the cost of living in the area.
> Entry supervisors earn $30+ an hour, but again, it is based on cost of living and work conditions. Reference labs pay more, but expect more.
>
> My first supervisor job paid $32, and I worked like crazy. It wasn't worth it.
> You get what you pay for though, and in this economic climate, the high salaries are not as plentiful as they once were.
>
> Toysha N. Mayer, MBA, HT (ASCP)
> Instructor
> Program in Histotechnology
> School of Health Professions
> MD Anderson Cancer Center
> (713) 563-3481
> tnmayer <@t> mdanderson.org
>
>
>
>
> Message: 6
> Date: Fri, 28 Oct 2011 12:52:23 -0400
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: RE: [Histonet] SALARY
> To: <kcastillo <@t> frii.com>,<Histonet <@t> lists.utsouthwestern.edu>,
> "Caroline Pratt" <Caroline.Pratt <@t> uphs.upenn.edu>
> Message-ID: <4EAAA586.7400.0077.1 <@t> harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> I think that's low. If you find a good candidate with years of experience I would pay them whatever it takes to get them in the door. It's like "Free Agency" in baseball; if you want a good player, you need to put the "money on table". Histotechnologists are the most valuable employees in the laboratory today!
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
> >>> "Pratt, Caroline" <Caroline.Pratt <@t> uphs.upenn.edu> 10/28/2011 9:21 AM >>>
> I would say $28 to $32 dollars an hour depending on experience and education.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kcastillo <@t> frii.com
> Sent: Friday, October 28, 2011 7:54 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] SALARY
>
> HI EVERYONE,
>
> WOULD LIKE TO KNOW WHAT LEAD TECHS AND SUPERVISORS ARE GETTING PAID
> THESE DAYS. HAVE A FELLOW HISTO PERSON THAT IS RUNNING THEIR OWN DERM
> LAB AND DOING MOHS ALSO. THANKS FOR YOUR HELP. KRISTY
>
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>
>
>
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> End of Histonet Digest, Vol 95, Issue 34
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------------------------------
Message: 17
Date: Mon, 31 Oct 2011 10:33:12 -0400 (EDT)
From: "Nicole Tatum" <nicole <@t> dlcjax.com>
Subject: [Histonet] background checks required by AHCA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1533.208.62.167.196.1320071592.squirrel <@t> webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1
I am currently filing out my ahca renewal application. There are many
changes and the form and I do not find it user friendly. Anyways here my
question: Medical directors and chief finicial officers or any person who
stands to gain profit must undergo a level 2 background screeening. On the
new app is states all empolyees and health care providers. I called acha
licensing division and they said only directors and so forth. But, on ahca
website statue 408(something, ill have to get exact number) when into
effect in 2010 that says all employees and newly hired employees must
underground background screening. So does all the arnp, pa, ht, and ma's
need to be screened because we have a lab?
Nicole Tatum, HT ASCP
http://ahca.myflorida.com/mchq/long_term_care/Background_Screening/BGS_WhoRequiredToBeScreened.pdf
taken directly from ahca site:
Employees and Contractors employed before August 1, 2010
Every employee/contractor must attest to meeting the requirements of this
chapter and agreeing to inform the employer immediately if arrested for
any of the disqualifying offenses while employed by the employer. [Section
435.05(2)]. This attestation must be maintained in the employee?s
personnel file. You may use the Affidavit of Compliance with Background
Screening to satisfy the attestation requirement.
If an employer becomes aware that an employee/contractor has been arrested
for a disqualifying offense, the employer must remove the
employee/contractor from contact with any vulnerable person that places
the employee/contractor in a role that requires background screening until
the arrest is resolved in a way that the employer determines that the
employee/contractor is still eligible for employment/contracting under
this chapter. [Section 435.06(2)(b)]
Rescreening
------------------------------
Message: 18
Date: Mon, 31 Oct 2011 10:56:17 -0500
From: "Orr, Rebecca" <ROrr <@t> northshore.org>
Subject: [Histonet] Dako Pr on Ventana?
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B44CA3426A2C084F9BA90B93D528159B05323AFF5D <@t> EXCH34.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
Hi Friends,
If anyone is using Dako PR concentrate clone 1294 on a Ventana, could you please contact me separately?
I'd like some advice.
Thank you
Becky
Becky Orr CLA,HT(ASCP)QIHC
Technical Specialist
Anatomic Pathology
NorthShore University HealthSystem
847-570-2771
-----Original Message-----
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