[Histonet] need help with TMA

[Patricia F Lott]

We have recently started doing tissue microarrays.  The problem we have is that some of the small cores drop out after the first few sections.

I think my problem is in the step where we are supposed to melt the donor and recipient blocks together.  We tried 5 minutes at 37 degrees, and then  minutes at 60 degrees x3, with cooling to room temp in between.

Any suggestions?

Thanks,
Patty Lott, UAB