[Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment

[Daniel Sjolander]

Why would you like to avoid detection of intracellular Abeta inclusions?

//Daniel

On Wed, Oct 26, 2011 at 8:51 AM, Hobbs, Carl <carl.hobbs <@t> kcl.ac.uk> wrote:

>
> Be careful: HIER may expose not only extracellular amyloid plaques but also
> intracellular APP ( or...is it intracellular A beta ???? ;-))
> Eg:  with 6E10.... it is directed against an epitope found in APP and also
> A beta: if you do Formic acid, only extracellular plaques are
> positive...after HIER, there is also intracellular positivity.
> In my experience.
> Some examples of this can be found here, in the image gallery :
> http://www.immunoportal.com/
>
>
> Whichever way you go....good luck.
>
> Carl
>
>
> Carl Hobbs
> Histology Manager
> Wolfson CARD
> School of Biomedical Sciences
> Kings College London
> Guys Campus
> SE1 1UL
> Tel: 020 78486813
> Fax: 020 78486816
> 020 78486813
>
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