[Histonet] RE: Histonet Digest, Vol 95, Issue 31
Alicia R. Lange
arlange <@t> medicine.nevada.edu
Thu Oct 27 11:46:00 CDT 2011
Hi Kevin,
I currently have the Leica IPS and IPC labeling system. There are some definitely pros/cons:
Pros:
Great for high volume
Has convenient tray loading attachment (optional)
Cons:
Takes up a lot of space
Very particular for type of slides used (may be a issue for cost saving purposes)
High costs for service plans, would have to compare prices to other models (pm's, maintenance etc)
Cassettes sometimes create static through the printer and prevent from ejecting out resulting in jamming
I have used this system in a low volume and high volume lab. They haven't given me too much trouble. The problems I have stated I have encountered within the 3 years I have been working with them.
Let me know if you need more information!
Alicia Lange
Laboratory Supervisor
University of Nevada, Reno
Pathology Department
arlange <@t> medicine.nevada.edu
------------------------------
Message: 2
Date: Tue, 25 Oct 2011 13:52:28 -0400
From: "Kevin Reif" <kreif <@t> stegh.on.ca>
Subject: [Histonet] Hi Group:
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4EA6BF1D.2151.00B6.0 <@t> stegh.on.ca>
Content-Type: text/plain; charset=US-ASCII
Hi Group:
Looking for opinions on cassette and slide labellers?? If you are using one please let me know the make, model and what you think of the machine, good or bad. Thanks in advance for all your input.
Kevin Reif
Senior Technologist - Pathology
St. Thomas-Elgin General Hospital
St. Thomas, On. Can.
__________________________________________________________________________
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Message: 3
Date: Tue, 25 Oct 2011 10:58:04 -0700 (PDT)
From: Steve Wong <swongman <@t> yahoo.com>
Subject: [Histonet] Please unsubscribe
To: Histonet <@t> lists.utsouthwestern.edu
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Hello,
Please unsubscribe me from this list.
swongman <@t> yahoo.com
Thanks!
------------------------------
Message: 4
Date: Tue, 25 Oct 2011 12:59:41 -0500
From: "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov>
Subject: [Histonet] saving slides between levels for IHC?
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>, "Horge,
Pamela J" <PHORGE1 <@t> Fairview.org>, <rollmann.denis <@t> mayo.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<DB425E28065DA14FAA75A282959620AF05ABD7B1 <@t> VHAV23MSGA2.v23.med.va.gov>
Content-Type: text/plain; charset="us-ascii"
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
------------------------------
Message: 5
Date: Tue, 25 Oct 2011 21:02:10 +0300
From: Badz Yahoo <badzrosari <@t> yahoo.com>
Subject: [Histonet] EMS 9000
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <80FD49AB-B170-4680-B36E-EEECD98F2800 <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi histonetts..Anyone using the microwave machine EMS 9000?I appreciate if someone can give me some feedback..tanx
------------------------------
Message: 6
Date: Tue, 25 Oct 2011 11:05:10 -0700
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: saving slides between levels for IHC?
To: "'Harrison, Sandra C.'" <Sandra.Harrison3 <@t> va.gov>, "Webb, Dorothy
L" <Dorothy.L.Webb <@t> HealthPartners.Com>, "Horge, Pamela J"
<PHORGE1 <@t> Fairview.org>, "rollmann.denis <@t> mayo.edu"
<rollmann.denis <@t> mayo.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8D7C2D242DBD45498006B21122072BF89448A30D <@t> MCINFRWEM003.ucsfmedicalcenter.org>
Content-Type: text/plain; charset=us-ascii
Sandy, we have various protocols, some that have unstained between those to be stained. However, we have moved away from having "extras" cut "just in case" because we had thousands of unstained slides stored that were never used. After a few months they may be unreliable for staining.
Tim Morken
Supervisor, Histology, IPOX
UCSF Medical Center
San Francisco, CA, USA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C.
Sent: Tuesday, October 25, 2011 11:00 AM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis <@t> mayo.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 25 Oct 2011 15:15:23 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] saving slides between levels for IHC?
To: "Pamela J Horge" <PHORGE1 <@t> Fairview.org>, "Dorothy L Webb"
<Dorothy.L.Webb <@t> HealthPartners.Com>, <rollmann.denis <@t> mayo.edu>,
"Sandra C. Harrison" <Sandra.Harrison3 <@t> va.gov>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4EA6D28B.2B7F.00C9.1 <@t> geisinger.edu>
Content-Type: text/plain; charset=US-ASCII
we do for certain part types. sentinel lymph nodes for melanoma, renal bxs, liver core bxs, prostate needle core bxs.
>>> "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov> 10/25/2011 1:59 PM >>>
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
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Message: 8
Date: Tue, 25 Oct 2011 21:44:11 +0100 (BST)
From: Rhonda Henshall-Powell <rlhenshall_powell <@t> yahoo.co.uk>
Subject: [Histonet] Seeking Field Application Specialists and
Education Coordinator for CA-based Cancer Diagnostics Company
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1319575451.47919.YahooMailNeo <@t> web29501.mail.ird.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello Histonetters!
Here I am again - An industry-leading IHC/ISH diagnostics company is seeking an in-house Education Coordinator and two Field Application Specialists (Midwest and Northwest) requiring significant travel:
Field Applications Specialist
Provide expert level technical support knowledge of Anatomic Pathology principles to troubleshoot and support instrumentation, software, and train lab personnel on use, optimisation and validation of IHC and ISH reagents.
If interested or know of someone who may be interested, please reply directly to me (rlhenshall_powell <@t> yahoo.co.uk) for more information.
Thank you
Best Regards,
Rhonda
------------------------------
Message: 9
Date: Tue, 25 Oct 2011 15:09:32 -0700
From: Patrick Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] saving slides between levels for IHC?
To: Angela Bitting <akbitting <@t> geisinger.edu>
Cc: Pamela J Horge <PHORGE1 <@t> fairview.org>, rollmann.denis <@t> mayo.edu,
Dorothy L Webb <Dorothy.L.Webb <@t> healthpartners.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAKEyg-2aEvCRc9iJs+QShs1ESMipvWwiuQ8_gS0janBS2eT5_g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We tend to take extras between levels especially on scant or needle biopsy
tissue. We do waste several thousand slides per year, but by doing this it
allows us to give the pathologist several representative sections and also
save tissue for future IHC and special stains.
On Tue, Oct 25, 2011 at 12:15 PM, Angela Bitting <akbitting <@t> geisinger.edu>wrote:
> we do for certain part types. sentinel lymph nodes for melanoma, renal bxs,
> liver core bxs, prostate needle core bxs.
>
> >>> "Harrison, Sandra C." <Sandra.Harrison3 <@t> va.gov> 10/25/2011 1:59 PM >>>
> Does anyone know if it is standard to save sections from between the
> levels for IP? Is it standard at your institution?
>
>
>
>
>
> Sandy C. Harrison, HTL (ASCP)
>
> Histology Supervisor
>
> Minneapolis VA
>
> 612-467-2449
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> IMPORTANT WARNING: The information in this message (and the documents
> attached to it, if any) is confidential and may be legally privileged. It is
> intended solely for the addressee. Access to this message by anyone else is
> unauthorized. If you are not the intended recipient, any disclosure,
> copying, distribution or any action taken, or omitted to be taken, in
> reliance on it is prohibited and may be unlawful. If you have received this
> message in error, please delete all electronic copies of this message (and
> the documents attached to it, if any), destroy any hard copies you may have
> created and notify me immediately by replying to this email. Thank you.
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> external e-mail messages. If email is encrypted, the recipient will receive
> an e-mail instructing them to sign on to the Geisinger Health System Secure
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--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com
------------------------------
Message: 10
Date: Wed, 26 Oct 2011 07:51:56 +0100
From: "Hobbs, Carl" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA
pretreatment
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<11D9615B89C10747B1C985966A63D7CA3826477A48 <@t> KCL-MAIL04.kclad.ds.kcl.ac.uk>
Content-Type: text/plain; charset="us-ascii"
Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-))
Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity.
In my experience.
Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/
Whichever way you go....good luck.
Carl
Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813
------------------------------
Message: 11
Date: Wed, 26 Oct 2011 07:28:54 -0400
From: "Sheila Fonner" <sfonner <@t> labpath.com>
Subject: RE: [Histonet] saving slides between levels for IHC?
To: "'Harrison, Sandra C.'" <Sandra.Harrison3 <@t> va.gov>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cc93d2$71a7f670$54f7e350$@com>
Content-Type: text/plain; charset="US-ASCII"
Sandy,
I don't think it is a "standard" procedure. However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted. We are a
dermatopathology lab. When it is requested, we cut the whole case on a
clean waterbath, using distilled water and no additives, on plus slides.
The routine H&E's are stained normally and the other slides are dried and
held for possible immunos.
Sheila
KDL Pathology
Knoxville, TN
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Tuesday, October 25, 2011 2:00 PM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis <@t> mayo.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 26 Oct 2011 09:42:18 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: Re: Seeking Beta-Amyloid not needing FA
pretreatment
To: "Hobbs, Carl" <carl.hobbs <@t> kcl.ac.uk>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE345DD4DF0E <@t> USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
Interesting....we see APP on frozen sections with 6E10, but not in paraffin sections after citrate HIER. We use the monoclonal from Covance, 1:12000 overnight at 4C.
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl
Sent: Wednesday, October 26, 2011 2:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Seeking Beta-Amyloid not needing FA pretreatment
Be careful: HIER may expose not only extracellular amyloid plaques but also intracellular APP ( or...is it intracellular A beta ???? ;-))
Eg: with 6E10.... it is directed against an epitope found in APP and also A beta: if you do Formic acid, only extracellular plaques are positive...after HIER, there is also intracellular positivity.
In my experience.
Some examples of this can be found here, in the image gallery : http://www.immunoportal.com/
Whichever way you go....good luck.
Carl
Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813
_______________________________________________
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Message: 13
Date: Wed, 26 Oct 2011 08:49:26 -0500
From: Roger Brown <rbrown <@t> mst.edu>
Subject: [Histonet] Please remove my name
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CACD7A16.92CD%rbrown <@t> mst.edu>
Content-Type: text/plain; charset="US-ASCII"
Hello,
Please remove my name from the Histonet mailing list.
Thank you,
Roger Brown (rbrown <@t> mst.edu)
------------------------------
Message: 14
Date: Wed, 26 Oct 2011 09:56:15 -0400
From: "Vanessa J. Phelan" <vjp2105 <@t> columbia.edu>
Subject: [Histonet] Please remove my name
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CACD89BF.6CF5%phelan <@t> icg.cpmc.columbia.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hi, please remove my name from the mailing list.
Thank you
------------------------------
Message: 15
Date: Wed, 26 Oct 2011 07:32:57 -0700
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: [Histonet] RE: Please remove my name
To: "'Roger Brown'" <rbrown <@t> mst.edu>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<493CAA64F203E14E8823737B9EE0E25F091E91273E <@t> EXCHMB01.isis.local>
Content-Type: text/plain; charset="us-ascii"
If you want to be removed from the list, you have to do it yourself by going to the website below.
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
1896 Rutherford Road
Carlsbad, CA 92008
760-603-2371
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roger Brown
Sent: Wednesday, October 26, 2011 6:49 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Please remove my name
Hello,
Please remove my name from the Histonet mailing list.
Thank you,
Roger Brown (rbrown <@t> mst.edu)
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------------------------------
Message: 16
Date: Wed, 26 Oct 2011 08:10:43 -0700 (PDT)
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] saving slides between levels for IHC?
To: Sheila Fonner <sfonner <@t> labpath.com>, "'Harrison, Sandra C.'"
<Sandra.Harrison3 <@t> va.gov>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1319641843.94105.YahooMailNeo <@t> web112302.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I think it depends on what the specimen is. All of the places Ive worked have always kept extras(levels) on sentinal nodes for keratins, also for prostate needle biopsies for p63/504s or other IHC's.
Really doesnt it come down to what the Pathologist needs to make a diagnosis for the patient?
I know it's a pain to cut so many levels and save them. Its also costly. But trust me, none of that is as bad as the sinking feeling you get when a biopsy has been exhausted and they need more stains :(
Hope this helps. Just my 2 cents worth.......
Kim Donadio
________________________________
From: Sheila Fonner <sfonner <@t> labpath.com>
To: "'Harrison, Sandra C.'" <Sandra.Harrison3 <@t> va.gov>; histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 26, 2011 7:28 AM
Subject: RE: [Histonet] saving slides between levels for IHC?
Sandy,
I don't think it is a "standard" procedure. However, our pathologist will
frequently ask us to keep intervening levels for possible IHC on certain
cases that are small or where the tumor is about to be exhausted. We are a
dermatopathology lab. When it is requested, we cut the whole case on a
clean waterbath, using distilled water and no additives, on plus slides.
The routine H&E's are stained normally and the other slides are dried and
held for possible immunos.
Sheila
KDL Pathology
Knoxville, TN
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Harrison,
Sandra C.
Sent: Tuesday, October 25, 2011 2:00 PM
To: Webb, Dorothy L; Horge, Pamela J; rollmann.denis <@t> mayo.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] saving slides between levels for IHC?
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Wed, 26 Oct 2011 12:00:59 -0400
From: "Sheila Fonner" <sfonner <@t> labpath.com>
Subject: [Histonet] EBER ISH
To: <histonet <@t> lists.utsouthwestern.edu>, "'Paul Googe'"
<pgooge <@t> labpath.com>, "'Susan Foreman'" <sforeman <@t> labpath.com>
Message-ID: <001901cc93f8$74289190$5c79b4b0$@com>
Content-Type: text/plain; charset="us-ascii"
Histonetters,
Is anyone out there using the EBER ISH probes from Ventana on the Ultra
Platform? I have a few questions. Did you need to get new software
installed? Was there any training involved? And do you have successful
protocols that you are willing to share?
I have asked the questions before, but at the time there was nobody actually
using it. I know that there were inquiries about the protocols because of
the ASR status and the fact that Ventana cannot give you any advice.
Any and all information would be greatly appreciated since we are wanting to
start it up at our facility.
Thanks,
Sheila
KDL Pathology
Knoxville, TN
------------------------------
Message: 18
Date: Wed, 26 Oct 2011 12:04:28 -0400
From: "Sheila Fonner" <sfonner <@t> labpath.com>
Subject: [Histonet] Antibody questions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001e01cc93f8$f085e120$d191a360$@com>
Content-Type: text/plain; charset="us-ascii"
Is there anyone who is doing myc, Bap1, Pten, or CD123 for dermpath
specimens, and who is your vendor? Do you like the products, and what
platform are you using?
Thanks for any information you may have. These are new requests from our
pathologist.
Sheila
------------------------------
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