[Histonet] ? on frozen tissue artifacts

[histopath101 <@t> gmail.com]

I never said these were muscles. These are tumors removed and frozen the  
day before and sent to us on dry ice. We know not to use frost-free  
freezers!

On , "Esparza, Sandra" <SEsparza <@t> seton.org> wrote:
> I have never put my muscles in a -20 we use -70 to -80 freezer and have


> not had a problem with artifact. Is your freezer frost free?





> Sandra


> Sandra Esparza HT(ASCP)QIHC


> Lead Technologist


> Dell Children's Medical Center of Central Texas


> 512-324-0000 x87061


> sesparza <@t> seton.org





> -----Original Message-----


> From: histonet-bounces <@t> lists.utsouthwestern.edu


> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of


> histopath101 <@t> gmail.com


> Sent: Friday, October 21, 2011 8:44 AM


> To: Histonet <@t> lists.utsouthwestern.edu


> Subject: [Histonet] ? on frozen tissue artifacts





> I work in a reference lab and some of our frozen sections exhibit severe





> freeze artifact (ice crystals). The client claims it's something we're


> doing and we say they are not freezing them properly. We receive the


> specimens on dry ice and store them in a -20C freezer until we section


> them


> (no more than 1-2 days later). We never allow the tissues to thaw. My


> question: Can freeze artifact occur AFTER properly snap-freezing tissue


> or


> does this artifact ONLY occur during the initial preezing procedure?


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