[Histonet] Fatty mamma tissue keeps on washing off

[Hoekert, W.E.J.]

Hi Histonetters,

Every once in a while, we have mamma tissue that washes off the slides completely. The reason for this is that the tissue is still too fat and/or not sufficiently fixed. There is no problem with the HE stain, but the immuno will wash off. What do you do in such cases?

I have tried the following:

1: Baking the slides for about 3/4 hours on superfrost slides (that works sometimes but not always)

2: Wood glue slides (that works sometimes but not always)

3:We put the blocks back in hot parraffin for several hours (that usually works) but I was told that this will have a bad influence on the Her2neu stain: a 2+ could become a 1+.

I know, the solution is to be sure that the tissue is fixed long enough and that the fat has been removed sufficiently using acetone, but sometimes that is just not the case. How do you cope with this?

Willem Hoekert
PA-lab
OLVG Amsterdam
The Netherlands



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