[Histonet] Annexin V IHC staining in mouse xenograft

[Jan Shivers]

Aprill,

If you send us a list of the exact reagents and incubation times/dilutions 
that you use, someone on Histonet may be able to answer your question.

Jan Shivers
Univ. of Minnesota

----- Original Message ----- 
From: <awatanabe <@t> tgen.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, October 11, 2011 12:11 PM
Subject: [Histonet] Annexin V IHC staining in mouse xenograft


Hi histonetters,
I need some help.  I am running an antibody from Biosensis called Annexin V 
in mouse xenograft brain samples with human glioma tumors treated and 
non-treated.  I have incredible background that I can not get rid of any of 
my samples.  I have used a protein block, rodent tissue block, post 
fixation, short antibody incubation time, short antigen retrieval time, very 
dilute antibody, polymer detection and every combination in between.  I'm 
looking to see if anyone knows anything else to try.  I'm trying to 
rearrange the protocol to put steps in different places to see if that 
works.  At this point I'm not sure what else to try so any ideas would be 
great.

Aprill Watanabe, B.S.
Research Associate II
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Macroanalyte Analysis & Processing Center (MAPC)
Translational Genomics Research Institute (TGen)
445 North 5th Street
Phoenix, AZ 85004
Main: 602-343-8822
Fax: 602-343-8717
Cell: 602-481-8654
email: awatanabe <@t> tgen.org
website: www.tgen.org

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