[Histonet] Imidazole buffer

[Geoff McAuliffe]

Hi Tammy:

Here are some references for imidazole in buffers:

Straus, W., 1982. Imidazole increases the sensitivity of the 
cytochemical reaction for peroxidase with diaminobenzidine at a neutral 
pH. J. Histochem. Cytochem. 30:491-493.

Trojanowski, J.Q., M.A. Obrocka and V. Lee. 1983. A comparison of eight 
different chromogen protocols for the demonstration of immunoreactive 
neurofilaments or glial filaments in rat cerebellum using the 
peroxidase-antiperoxidase method and monoclonal antibodies. J. 
Histochem. Cytochem. 31:1217-1223.

Geoff


On 10/6/2011 11:49 AM, Tammy Pickles wrote:
> Hi,
>
> I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share?
>
> Thanks in advance,
> Tammy
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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