[Histonet] Imidazole buffer

Tammy Pickles Tammy.Pickles <@t> inspection.gc.ca
Thu Oct 6 10:49:08 CDT 2011


Hi,
 
I am wondering if anyone out there can tell me the function of Imidazole, when used in a buffer. We are currently using it to make up our in house DAB solution. The results are fantastic with an overall increase in sensitivity with all of our antibodies. The only information I can find concerning the function is to help in blocking nonspecific staining. Does anyone have more information to share?
 
Thanks in advance,
Tammy


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