[Histonet] Green counterstain problems

[Bell, Lynne]

We have had this problem before.  Make sure that the glacial acetic acid has been added to the light green stock solution.  Cured our problem!!!

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT  05641
802-371-4923
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of rgrow <@t> bmnet.com
Sent: Tuesday, October 04, 2011 10:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Green counterstain problems


Good Morning Histonetters!

I've been having a counterstain problem with my GMS and PAS-F.  Both stains
work as they should until the green counterstain is applied.  I cannot get
my tissue (control or patient) to uptake the counterstain.  I have varied
time, temperature, even companies.  I have good controls that have been in
use for years without problems, until now.  Only after an extended -10+
minutes,- can I get a blush of color in them.  I've even called the slide
company to ask if there has been a change to the charge coating
I'm open to suggestions.
Thanks!

Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax
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