[Histonet] Green counterstain problems

[rgrow <@t> bmnet.com]

Good Morning Histonetters!

I've been having a counterstain problem with my GMS and PAS-F.  Both stains
work as they should until the green counterstain is applied.  I cannot get
my tissue (control or patient) to uptake the counterstain.  I have varied
time, temperature, even companies.  I have good controls that have been in
use for years without problems, until now.  Only after an extended -10+
minutes,- can I get a blush of color in them.  I've even called the slide
company to ask if there has been a change to the charge coating
I'm open to suggestions.
Thanks!

Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax
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