[Histonet] decal question

Houston, Ronald Ronald.Houston <@t> nationwidechildrens.org
Mon Oct 3 13:25:32 CDT 2011


Alcoholic formalin or 70% ethanol as, quoting Gayle Callis, "alcohol can slow or prevent decalcification"

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Monday, October 03, 2011 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decal question

Hi Everyone,

 

I have a new student taking course work at UND and using my lab for her practical clinical site.  She took a test on decal today and there was a question we didn't know the answer to.

 

What fixative should not be used for acid decalcification?  Would it be osmium tetroxide?

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net web site  <http://www.ihctech.net> www.ihctech.net

 


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