[Histonet] Re: Histonet Digest, Vol 96, Issue 42
M. Kap
m.kap.1 <@t> erasmusmc.nl
Wed Nov 30 16:45:04 CST 2011
@15
Wrap tissue in moist, not too wet!!, (0.9% salt or PBS) gauze and store
at 4 degrees C. No/hardly any harm done to morphology, antigenicity, RNA,
DNA and so on. Tissue is tougher than you think...
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027704
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. nuclear fast red (Patsy Ruegg)
> 2. RE: nuclear fast red (Elizabeth Chlipala)
> 3. Histologist needed in Austin Texas (Esparza, Sandra)
> 4. validation (Amos Brooks)
> 5. autostainer vials (Thomas Pier)
> 6. Diane Tokugawa/CA/KAIPERM is out of the office.
> (Diane.Tokugawa <@t> kp.org)
> 7. Question regarding cutting on microtome (Marcia Spencer)
> 8. RE: Question regarding cutting on microtome (Britton, Josette C)
> 9. Chameleon tissue lifting off (Thomas, Nancy)
> 10. (no subject) (JThawley <@t> ShoreMemorial.org)
> 11. RE: (no subject) (Rathborne, Toni)
> 12. RE: Chameleon tissue lifting off (WILLIAM DESALVO)
> 13. Immunohistochemical staining of type IV collagen in Alport's
> Syndrome (CHRISTIE GOWAN)
> 14. Part-time Job (Maria T)
> 15. fresh specimens after hours (Houston, Ronald)
> 16. RE: fresh specimens after hours (joelle weaver)
> 17. ASR FISH probes (Martin, Erin)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 29 Nov 2011 11:29:07 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: [Histonet] nuclear fast red
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <94C3B2CA09434506BBEB3246C1D686EB <@t> Patsyoffice>
> Content-Type: text/plain; charset="us-ascii"
>
> Is my nuclear fast red powder dead or does it take a long time with heat
> and
> stirring to turn red? It is pretty old, and one vial says it should be
> stored below 0 but has not been.
>
>
>
> Cheers,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
> web site <http://www.ihctech.net> www.ihctech.net
>
>
>
>
> This email is confidential and intended solely for the use of the
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that
> is
> privileged & confidential within the meaning of applicable law.
> Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited.
> If
> you are NOT the intended recipient please contact the sender and dispose
> of
> this e-mail as soon as possible.
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 29 Nov 2011 13:22:21 -0700
> From: Elizabeth Chlipala <liz <@t> premierlab.com>
> Subject: RE: [Histonet] nuclear fast red
> To: Patsy Ruegg <pruegg <@t> ihctech.net>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <14E2C6176416974295479C64A11CB9AE011380AD9C8D <@t> SBS2K8.premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Patsy
>
> It does not go completely in solution we filter prior to staining
>
> Liz
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
> [pruegg <@t> ihctech.net]
> Sent: Tuesday, November 29, 2011 11:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] nuclear fast red
>
> Is my nuclear fast red powder dead or does it take a long time with heat
> and
> stirring to turn red? It is pretty old, and one vial says it should be
> stored below 0 but has not been.
>
>
>
> Cheers,
>
> Patsy
>
>
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net
> web site <http://www.ihctech.net> www.ihctech.net
>
>
>
>
> This email is confidential and intended solely for the use of the
> Person(s)
> ('the intended recipient') to whom it was addressed. Any views or opinions
> presented are solely those of the author. It may contain information that
> is
> privileged & confidential within the meaning of applicable law.
> Accordingly
> any dissemination, distribution, copying, or other use of this message, or
> any of its contents, by any person other than the intended recipient may
> constitute a breach of civil or criminal law and is strictly prohibited.
> If
> you are NOT the intended recipient please contact the sender and dispose
> of
> this e-mail as soon as possible.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 29 Nov 2011 14:52:25 -0600
> From: "Esparza, Sandra" <SEsparza <@t> seton.org>
> Subject: [Histonet] Histologist needed in Austin Texas
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3D79F47DC92B204F9E5D35C885DFC5CB041DFE7F <@t> AUSEX2VS1.seton.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Full Time/Day Shift position open for a HT/HTL (ASCP) registered
> histologist, with 2+ years working experience. Requires strong
> embedding and microtomy skills, knowledge of muscle enzymes, EM, and IHC
> is preferred. Proficiency in these areas can be obtained on the job. We
> are a highly Specialized Histology department with state of the art
> equipment. This position is at a Trauma 1 children's hospital located in
> beautiful progressive Austin Texas. If you are interested in working in
> a team environment please go to Seton.net to apply online.
>
>
>
>
>
> Sandra Esparza HT(ASCP)QIHC
>
> sesparza <@t> seton.org
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 29 Nov 2011 16:24:28 -0500
> From: Amos Brooks <amosbrooks <@t> gmail.com>
> Subject: [Histonet] validation
> To: histonet <@t> lists.utsouthwestern.edu, amber.mckenzie <@t> gastrodocs.net
> Message-ID:
> <CAC95ki9+nVq4qLSjkARo9NTKEhQLdSos3jEfcYL2yH_yB5ytUQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
> I would say no, and if any inspector disagreed you would be well
> within
> your rights to give him a good cuff upside the head. The point in having a
> negative mouse and rabbit is to run them at the same concentration as
> whatever primary you are running. That would mean you would need to
> validate it at every conceivable dilution you could ever have a primary
> antibody at. Kinda silly if you think about it. Any good validation for a
> primary antibody should include a proper isotype negative control anyway,
> but that is a separate can o' worms.
>
> Amos
>
>
> On Tue, Nov 29, 2011 at 1:01 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
>> Message: 5
>> Date: Mon, 28 Nov 2011 20:00:03 +0000
>> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
>> Subject: [Histonet] validation
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <5A33C952BB67F4468AF1F36D739212BC0642BE <@t> JERRY.Gia.com>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Do you have to validate the neg mouse and rabbit?
>>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 29 Nov 2011 16:30:17 -0600
> From: "Thomas Pier" <tp2 <@t> medicine.wisc.edu>
> Subject: [Histonet] autostainer vials
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <4ED5089A020000DF0000C9DF <@t> gwmail.medicine.wisc.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> I'm using a Labvision Autostainer 360. I typically wash and reuse the
> reagent vials. Does anybody out there have any idea how long the vials
> will last doing this? Is there anything I'm not thinking of that I should
> be concerned about?
>
> Tom
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 29 Nov 2011 15:02:52 -0800
> From: Diane.Tokugawa <@t> kp.org
> Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OF2E5018C0.87390985-ON88257957.007E9AF3-88257957.007E9AF3 <@t> kp.org>
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting 11/29/2011 and will not return until
> 12/05/2011.
>
> Note: For Cytology issues, please call Molly at 8-421-5487, Eric at
> 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario
> at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404, or
> Wanda at 8-421-5426.
>
> ------------------------------
>
> Message: 7
> Date: Tue, 29 Nov 2011 22:49:10 -0500
> From: Marcia Spencer <marcia.spencer26 <@t> gmail.com>
> Subject: [Histonet] Question regarding cutting on microtome
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAMA_98yr-iHRPiwVUbdcW2io98RxM7M6jCHodgSBfvSwe2tagQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> I am wondering what causes a "ribbon" appearance in skin when viewed
> under the microscope, usually seen in the epidermis, but occasionally
> can be seen in the underlying tissue as well. I find this effect
> happens only in a few, 4 or 5 slides out of 100, but, I don't know how
> to correct it. I would describe it as the "old fashion hard candy
> that was rippled like a ribbon could be. Under the scope, focusing up
> and down demonstrates that the section is complete, just not on the
> same plane. Any suggestions?
> Thank you for your time
> M. Spencer
>
> On 11/27/11, histonet-request <@t> lists.utsouthwestern.edu
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>> Send Histonet mailing list submissions to
>> histonet <@t> lists.utsouthwestern.edu
>>
>> To subscribe or unsubscribe via the World Wide Web, visit
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> or, via email, send a message with subject or body 'help' to
>> histonet-request <@t> lists.utsouthwestern.edu
>>
>> You can reach the person managing the list at
>> histonet-owner <@t> lists.utsouthwestern.edu
>>
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>>
>>
>> Today's Topics:
>>
>> 1. dako autostainer labeler (Patsy Ruegg)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Sun, 27 Nov 2011 08:55:58 -0700
>> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
>> Subject: [Histonet] dako autostainer labeler
>> To: <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <B29772909A06463CB04824EF6F2F4687 <@t> prueggihctechlt>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> Greetings,
>>
>>
>>
>> I am looking for another Seymour labeler like the one used on the Dako
>> Autostainer, does anyone have one sitting around not being used I could
>> purchase from you? It needs to have the software disc so I can install
>> it
>> on a computer.
>>
>>
>>
>> Regards,
>>
>>
>>
>> Patsy
>>
>>
>>
>> Patsy Ruegg, HT(ASCP)QIHC
>>
>> IHCtech
>>
>> 12635 Montview Blvd. Ste.215
>>
>> Aurora, CO 80045
>>
>> 720-859-4060
>>
>> fax 720-859-4110
>>
>> www.ihctech.net
>>
>> www.ihcrg.org
>>
>>
>>
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 96, Issue 39
>> ****************************************
>>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 30 Nov 2011 09:08:42 -0500
> From: "Britton, Josette C" <JCBRITTON <@t> Cheshire-Med.COM>
> Subject: RE: [Histonet] Question regarding cutting on microtome
> To: "Marcia Spencer" <marcia.spencer26 <@t> gmail.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <F644CD64B2313F43BB3D2496B454CDAC038C2478 <@t> CMC-EX01.cheshire-med.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Your waterbath is not hot enough!
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia
> Spencer
> Sent: Tuesday, November 29, 2011 10:49 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Question regarding cutting on microtome
>
>
>
> I am wondering what causes a "ribbon" appearance in skin when viewed
>
> under the microscope, usually seen in the epidermis, but occasionally
>
> can be seen in the underlying tissue as well. I find this effect
>
> happens only in a few, 4 or 5 slides out of 100, but, I don't know how
>
> to correct it. I would describe it as the "old fashion hard candy
>
> that was rippled like a ribbon could be. Under the scope, focusing up
>
> and down demonstrates that the section is complete, just not on the
>
> same plane. Any suggestions?
>
> Thank you for your time
>
> M. Spencer
>
>
>
> On 11/27/11, histonet-request <@t> lists.utsouthwestern.edu
>
> <histonet-request <@t> lists.utsouthwestern.edu> wrote:
>
>> Send Histonet mailing list submissions to
>
>> histonet <@t> lists.utsouthwestern.edu
>
>>
>
>> To subscribe or unsubscribe via the World Wide Web, visit
>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>> or, via email, send a message with subject or body 'help' to
>
>> histonet-request <@t> lists.utsouthwestern.edu
>
>>
>
>> You can reach the person managing the list at
>
>> histonet-owner <@t> lists.utsouthwestern.edu
>
>>
>
>> When replying, please edit your Subject line so it is more specific
>
>> than "Re: Contents of Histonet digest..."
>
>>
>
>>
>
>> Today's Topics:
>
>>
>
>> 1. dako autostainer labeler (Patsy Ruegg)
>
>>
>
>>
>
>> ----------------------------------------------------------------------
>
>>
>
>> Message: 1
>
>> Date: Sun, 27 Nov 2011 08:55:58 -0700
>
>> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
>
>> Subject: [Histonet] dako autostainer labeler
>
>> To: <histonet <@t> lists.utsouthwestern.edu>
>
>> Message-ID: <B29772909A06463CB04824EF6F2F4687 <@t> prueggihctechlt>
>
>> Content-Type: text/plain; charset="us-ascii"
>
>>
>
>> Greetings,
>
>>
>
>>
>
>>
>
>> I am looking for another Seymour labeler like the one used on the Dako
>
>> Autostainer, does anyone have one sitting around not being used I
> could
>
>> purchase from you? It needs to have the software disc so I can
> install it
>
>> on a computer.
>
>>
>
>>
>
>>
>
>> Regards,
>
>>
>
>>
>
>>
>
>> Patsy
>
>>
>
>>
>
>>
>
>> Patsy Ruegg, HT(ASCP)QIHC
>
>>
>
>> IHCtech
>
>>
>
>> 12635 Montview Blvd. Ste.215
>
>>
>
>> Aurora, CO 80045
>
>>
>
>> 720-859-4060
>
>>
>
>> fax 720-859-4110
>
>>
>
>> www.ihctech.net
>
>>
>
>> www.ihcrg.org
>
>>
>
>>
>
>>
>
>>
>
>>
>
>> ------------------------------
>
>>
>
>> _______________________________________________
>
>> Histonet mailing list
>
>> Histonet <@t> lists.utsouthwestern.edu
>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>>
>
>> End of Histonet Digest, Vol 96, Issue 39
>
>> ****************************************
>
>>
>
>
>
> _______________________________________________
>
> Histonet mailing list
>
> Histonet <@t> lists.utsouthwestern.edu
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 9
> Date: Wed, 30 Nov 2011 09:00:49 -0600
> From: "Thomas, Nancy" <nto <@t> stowers.org>
> Subject: [Histonet] Chameleon tissue lifting off
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <2C40E43D1F7A56408C4463FD245DDDF993990281 <@t> EXCHMB-02.stowers-institute.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I would like to ask anyone who sections chameleon tissue what type of
> slides they use. I was having too much lifting while using the superfrost
> plus slides. It looked like slides coated with Haupt's solution were
> highly recommended, so I tried that. It is so much better, but still
> there is some lifting. If someone is successful with sectioning and
> staining lizard tissue without lifting, please advise me on type of
> slides, drying times, or anything that might help.
> Thank you so much,
>
> Nancy Thomas
> Stowers Institute for Medical Research
> Kansas City, MO
>
>
> ------------------------------
>
> Message: 10
> Date: Wed, 30 Nov 2011 10:19:46 -0500
> From: JThawley <@t> ShoreMemorial.org
> Subject: [Histonet] (no subject)
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFE19CEFA6.CC2ACFAA-ON85257958.0053ECE9-85257958.00543528 <@t> shoremedicalcenter.org>
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> Hello All,
>
> We are having a issue with our intercom system in the OR so the
> pathologist
> are having issues communicating frozen section diagnosis with the surgeon.
> Does anyone in Histoland use a written form to send frozen section
> diagnosis? Per CAP if it is verbal the pathologist must speak directly
> with
> the surgeon. Any suggestions would be much appreciated.
>
>
> Jennifer Thawley HT, ASCP
> Histology Supervisor
> Shore Memorial Hospital
> (609) 653-3940
>
>
> This transmittal from Shore Memorial Health System is for the sole use of
> the intended recipient and may contain confidential and privileged
> information. Any unauthorized review or use, including disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender and destroy all copies of the transmittal.
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Wed, 30 Nov 2011 15:56:19 +0000
> From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
> Subject: RE: [Histonet] (no subject)
> To: "'JThawley <@t> ShoreMemorial.org'" <JThawley <@t> ShoreMemorial.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3AD061FE740D464FAC7BF6B5CFB7570711F62FCD <@t> SMCMAIL01.somerset-healthcare.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We switched from intercoms to phones some years back. The phone is put on
> speaker mode in the OR. I suppose problems can occur with the phone system
> too, but we haven't experienced this.
> Regarding the CAP part of the question, I would call them. Ask if you can
> send a written diagnosis, which you are to receive back with the
> physician's/designee's signature. You can then document the intercom
> problems, and how you worked around it.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> JThawley <@t> ShoreMemorial.org
> Sent: Wednesday, November 30, 2011 10:20 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> Hello All,
>
> We are having a issue with our intercom system in the OR so the
> pathologist are having issues communicating frozen section diagnosis with
> the surgeon.
> Does anyone in Histoland use a written form to send frozen section
> diagnosis? Per CAP if it is verbal the pathologist must speak directly
> with the surgeon. Any suggestions would be much appreciated.
>
>
> Jennifer Thawley HT, ASCP
> Histology Supervisor
> Shore Memorial Hospital
> (609) 653-3940
>
>
> This transmittal from Shore Memorial Health System is for the sole use of
> the intended recipient and may contain confidential and privileged
> information. Any unauthorized review or use, including disclosure or
> distribution is prohibited. If you are not the intended recipient, please
> contact the sender and destroy all copies of the transmittal.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Somerset Medical Center
> and are intended only for the addressee. The information contained in
> this
> message is confidential and may contain privileged, confidential,
> proprietary and/or trade secret information entitled to protection and/or
> exemption from disclosure under applicable law. Unauthorized forwarding,
> printing, copying, distribution, or use of such information is strictly
> prohibited and may be unlawful. If you are not the addressee, please
> promptly delete this message and notify the sender of the delivery error
> by e-mail or you may call Somerset Medical Center's computer Help Desk
> at 908-685-2200, ext. 4050.
>
> Be sure to visit Somerset Medical Center's Web site -
> www.somersetmedicalcenter.com - for the most up-to-date news,
> event listings, health information and more.
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 30 Nov 2011 09:29:37 -0700
> From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
> Subject: RE: [Histonet] Chameleon tissue lifting off
> To: <nto <@t> stowers.org>, histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY151-W315A9BC9A8C926AAE0076A91B00 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> I worked w/ animal tissue, not specifically lizard, several years ago and
> we always used Poly-L-Lysine coated slides. I found the polycationic
> nature of this molecule seemed to interact well w/ non-human species and
> created a strong interaction with the anionic sites of animal tissue
> sections. Poly-L-Lysine always produced strong adhesive properties. We
> also dried the sections at room temperature for 24 hours as we found that
> longer and slower worked best for us.
>
> William DeSalvo, B.S., HTL(ASCP)
>
>
>
>> From: nto <@t> stowers.org
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 30 Nov 2011 09:00:49 -0600
>> Subject: [Histonet] Chameleon tissue lifting off
>>
>> I would like to ask anyone who sections chameleon tissue what type of
>> slides they use. I was having too much lifting while using the
>> superfrost plus slides. It looked like slides coated with Haupt's
>> solution were highly recommended, so I tried that. It is so much better,
>> but still there is some lifting. If someone is successful with
>> sectioning and staining lizard tissue without lifting, please advise me
>> on type of slides, drying times, or anything that might help.
>> Thank you so much,
>>
>> Nancy Thomas
>> Stowers Institute for Medical Research
>> Kansas City, MO
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 30 Nov 2011 16:51:18 +0000
> From: CHRISTIE GOWAN <christiegowan <@t> msn.com>
> Subject: [Histonet] Immunohistochemical staining of type IV collagen
> in Alport's Syndrome
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT122-W21F8C48A161E5469E7AAB2AEB00 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> We are looking into the feasability of doing this test in-house. Is anyone
> doing this test for kidney and if so, how did you run your validation? We
> have some positive patients but they are all skin so I guess we will need
> to purchase controls specifically for kidney. If anyone has any experience
> with this I would appreciate your comments. Thanks.
> Christie Gowan
> UAB University Hospital
>
> ------------------------------
>
> Message: 14
> Date: Wed, 30 Nov 2011 08:56:17 -0800 (PST)
> From: Maria T <histology.houston <@t> yahoo.com>
> Subject: [Histonet] Part-time Job
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1322672177.64453.YahooMailNeo <@t> web140502.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Needed a PRN for a small GI Lab in Houston… The position is one week a
> month from 2-10pm depending on workload.Â
> Position includes grossing BX’s, processing, embedding and cutting.Â
> If interested in position please email your resume along with any
> questions.
>
> Thanks
>
>
> ------------------------------
>
> Message: 15
> Date: Wed, 30 Nov 2011 17:10:12 +0000
> From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
> Subject: [Histonet] fresh specimens after hours
> To: 'histo net' <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <E5D0CD2352E46545A0C0EBE308CCCE53191770 <@t> L1PERDWXMB01.childrensroot.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> for facilities that are NOT staffed 24/7, how are fresh specimens handled
> after hours?
>
> Up till now, OR staff have called the pathologist on-call to determine how
> best to handle the specimen, but that process has broken down on the OR
> side of things. The OR Director does not want 2 different ways of dealing
> with fresh specimens and they now want a process that will be the same
> 24/7.
>
> As we will not be staffing the lab 24/7 on the odd chance a fresh specimen
> might come, I am interested in what other facilities may be doing.
>
> Thanks
> Ronnie
>
>
> Ronnie Houston, MS HT(ASCP)QIHC
> Anatomic Pathology Manager
> ChildLab, a Division of Nationwide Children's Hospital
> www.childlab.com
>
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
> www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
>
> "One person with passion is better than forty people merely interested."
> ~ E.M. Forster
>
>
>
> ----------------------------------------- Confidentiality Notice:
> The following mail message, including any attachments, is for the
> sole use of the intended recipient(s) and may contain confidential
> and privileged information. The recipient is responsible to
> maintain the confidentiality of this information and to use the
> information only for authorized purposes. If you are not the
> intended recipient (or authorized to receive information for the
> intended recipient), you are hereby notified that any review, use,
> disclosure, distribution, copying, printing, or action taken in
> reliance on the contents of this e-mail is strictly prohibited. If
> you have received this communication in error, please notify us
> immediately by reply e-mail and destroy all copies of the original
> message. Thank you.
>
> ------------------------------
>
> Message: 16
> Date: Wed, 30 Nov 2011 17:23:00 +0000
> From: joelle weaver <joelleweaver <@t> hotmail.com>
> Subject: RE: [Histonet] fresh specimens after hours
> To: <ronald.houston <@t> nationwidechildrens.org>, Histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <SNT135-W13A766857351C3770279AD8B00 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> RonnieFrom my experience when working in labs that are not staffed 24/7
> with HT or grossing personnel, after hours fresh specimens are called to a
> pathologist on call by the OR staff, who may then make the judgment to
> instruct them on fixation needed, or come in for a FS or view the
> specimen. Most places have used a pager or page # system with an on call
> rotation. Sometimes a histology person has been on -call after hours too (
> if they usually do the FS, accessioning etc at the bench in lieu of PA) or
> just be available certain hours if the pathologist needs them to assist
> with FS, other preparations for send outs etc.I think this is pretty much
> what you have suggested, and that seems to be in alignment with the
> arrangments and expectations I have worked for these circumstances in a
> few of my positions without 24 hr staff.Joelle
>
> Joelle Weaver MAOM, BA, (HTL) ASCP
>
> http://www.linkedin.com/in/joelleweaver
>
> > From: Ronald.Houston <@t> nationwidechildrens.org
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Wed, 30 Nov 2011 17:10:12 +0000
>> Subject: [Histonet] fresh specimens after hours
>>
>> for facilities that are NOT staffed 24/7, how are fresh specimens
>> handled after hours?
>>
>> Up till now, OR staff have called the pathologist on-call to determine
>> how best to handle the specimen, but that process has broken down on the
>> OR side of things. The OR Director does not want 2 different ways of
>> dealing with fresh specimens and they now want a process that will be
>> the same 24/7.
>>
>> As we will not be staffing the lab 24/7 on the odd chance a fresh
>> specimen might come, I am interested in what other facilities may be
>> doing.
>>
>> Thanks
>> Ronnie
>>
>>
>> Ronnie Houston, MS HT(ASCP)QIHC
>> Anatomic Pathology Manager
>> ChildLab, a Division of Nationwide Children's Hospital
>> www.childlab.com
>>
>> 700 Children's Drive
>> Columbus, OH 43205
>> (P) 614-722-5450
>> (F) 614-722-2899
>> ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
>> www.NationwideChildrens.org<http://www.nationwidechildrens.org/>
>>
>> "One person with passion is better than forty people merely interested."
>> ~ E.M. Forster
>>
>>
>>
>> ----------------------------------------- Confidentiality Notice:
>> The following mail message, including any attachments, is for the
>> sole use of the intended recipient(s) and may contain confidential
>> and privileged information. The recipient is responsible to
>> maintain the confidentiality of this information and to use the
>> information only for authorized purposes. If you are not the
>> intended recipient (or authorized to receive information for the
>> intended recipient), you are hereby notified that any review, use,
>> disclosure, distribution, copying, printing, or action taken in
>> reliance on the contents of this e-mail is strictly prohibited. If
>> you have received this communication in error, please notify us
>> immediately by reply e-mail and destroy all copies of the original
>> message. Thank you.
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 30 Nov 2011 17:55:14 +0000
> From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
> Subject: [Histonet] ASR FISH probes
> To: histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <24B7B291CC88D04AB663958E77A1F59D0133E7 <@t> ex09.net.ucsf.edu>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi all,
>
>
>
> More questions on FISH. How does one go about validating an ASR probe on
> FFPE tissue? I have no FISH experience and therefore am starting from
> scratch. The specific probes that our pathologists are interested in are:
> t(14;18), t(17;22) and t(2;5).
>
>
>
> Also, does anyone know what educational and training background is
> required to be able to do such a workup? I know IVDs are no problems, I
> looked at the CAP checklist for molecular and it seems that this is
> usually done in cytogenetics and the tech needs to at least have a BS and
> experience with molecular.
>
>
>
> Any help would be greatly appreciated!
>
>
>
> Thanks,
>
> Erin
>
>
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 96, Issue 42
> ****************************************
>
More information about the Histonet
mailing list