[Histonet] Question regarding cutting on microtome

[Britton, Josette C]

Your waterbath is not hot enough!

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marcia
Spencer
Sent: Tuesday, November 29, 2011 10:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question regarding cutting on microtome

 

I am wondering what causes a "ribbon" appearance in skin when viewed

under the microscope, usually seen in the epidermis, but occasionally

can be seen in the underlying tissue as well.  I find this effect

happens only in a few, 4 or 5 slides out of 100, but, I don't know how

to correct it.  I would describe it as the "old fashion hard candy

that was rippled like a ribbon could be.  Under the scope, focusing up

and down demonstrates that the section is complete, just not on the

same plane.  Any suggestions?

Thank you for your time

M. Spencer

 

On 11/27/11, histonet-request <@t> lists.utsouthwestern.edu

<histonet-request <@t> lists.utsouthwestern.edu> wrote:

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>    1. dako autostainer labeler (Patsy Ruegg)

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> ----------------------------------------------------------------------

> 

> Message: 1

> Date: Sun, 27 Nov 2011 08:55:58 -0700

> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>

> Subject: [Histonet] dako autostainer labeler

> To: <histonet <@t> lists.utsouthwestern.edu>

> Message-ID: <B29772909A06463CB04824EF6F2F4687 <@t> prueggihctechlt>

> Content-Type: text/plain;          charset="us-ascii"

> 

> Greetings,

> 

> 

> 

> I am looking for another Seymour labeler like the one used on the Dako

> Autostainer, does anyone have one sitting around not being used I
could

> purchase from you?  It needs to have the software disc so I can
install it

> on a computer.

> 

> 

> 

> Regards,

> 

> 

> 

> Patsy

> 

> 

> 

> Patsy Ruegg, HT(ASCP)QIHC

> 

> IHCtech

> 

> 12635 Montview Blvd. Ste.215

> 

> Aurora, CO 80045

> 

> 720-859-4060

> 

> fax 720-859-4110

> 

> www.ihctech.net

> 

> www.ihcrg.org

> 

> 

> 

> 

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> End of Histonet Digest, Vol 96, Issue 39

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