[Histonet] validation

[Rene J Buesa]

I have been thinking about your question and it seems to me that it is similar to proving a negative or a non existent situation or entity. Proving the non-existence of something is impossible the same as proving the existence of something non-existent.
On the other hand, you may have a protocol so "off" that you could have a heavy background in a supposedly non reacting section, and you could try to adjust it to make sure that the "non-reactive" tissue should just be that, negative.
You could run your negative through the whole protocol and compare its "negativity" against a section that has NOT been run through your protocol, just hematoxylin stained, and see if both are equal.
Other than that I do not think "validating a negative" is worth much thought or effort.
I never did it, and I do not see the advantage of doing it, although I may be wrong (as in many an occasion).
René J.

--- On Mon, 11/28/11, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:


From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] validation
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, November 28, 2011, 3:00 PM


Do you have to validate the neg mouse and rabbit?


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