[Histonet] demonstration of asbestos by means of electron microscopy

[Lee & Peggy Wenk]

The StainsFile page has some techniques, using polarizing microscopes.

http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm

Asbestos are usually very small fibers, and will not show up in every 
section. Cutting thicker sections sometimes helps.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message----- 
From: Yolanda Davies
Sent: Tuesday, November 22, 2011 4:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] demonstration of asbestos by means of electron 
microscopy

Dear all

I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.

Is it possible to reveal asbestos by means of electron microscopy?

Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the
asbestos?

Thank you in advance

Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Observatory
Cape Town
South Africa




###

UNIVERSITY OF CAPE TOWN

This e-mail is subject to the UCT ICT policies and e-mail disclaimer
published on our website at
http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from
+27 21 650 9111. This e-mail is intended only for the person(s) to whom
it is addressed. If the e-mail has reached you in error, please notify
the author. If you are not the intended recipient of the e-mail you may
not use, disclose, copy, redirect or print the content. If this e-mail
is not related to the business of UCT it is sent by the sender in the
sender's individual capacity.

###







_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet