[Histonet] demonstration of asbestos by means of electron microscopy

Lee & Peggy Wenk lpwenk <@t> sbcglobal.net
Tue Nov 22 03:56:40 CST 2011

The StainsFile page has some techniques, using polarizing microscopes.


Asbestos are usually very small fibers, and will not show up in every 
section. Cutting thicker sections sometimes helps.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message----- 
From: Yolanda Davies
Sent: Tuesday, November 22, 2011 4:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] demonstration of asbestos by means of electron 

Dear all

I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not

Is it possible to reveal asbestos by means of electron microscopy?

Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the

Thank you in advance

Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Cape Town
South Africa



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