[Histonet] demonstration of asbestos by means of electron microscopy

[Yolanda Davies]

Dear all
 
I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.
 
Is it possible to reveal asbestos by means of electron microscopy?
 
Usually asbestos is demonstrated using the Perl's Prussian blue
technique, but most times they are elusive.
Could it be because of the sampling site or simply the nature of the
asbestos?
 
Thank you in advance
 
Yolanda Davies
Department of Forensic Medicine and Toxicology
Falmouth building
Anzio Road
Observatory
Cape Town 
South Africa




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