[Histonet] Re: counterstain for fast red
Bob Richmond
rsrichmond <@t> gmail.com
Tue Nov 15 13:23:05 CST 2011
In choosing a nuclear stain color I'd want to know if the pathologist
has normal color vision - remember one man in eight doesn't. Green
would not be a good counterstain for red IHC precipitate in a man with
red-green color blindness. I'd ask him.
The most serious problem I've seen with color-blind pathologists is
that they can't read traditional light-microscopic acid-fast stains,
with red bugs and blue background. There are work-arounds, but it's
much better to do it by fluorescence (auramine O) or find a reference
lab that can.
Bob Richmond
Samurai Pathologist (with normal color vision, though some who know my
taste in clothing have disputed that)
Knoxville TN
On Tue, Nov 15, 2011 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
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> Today's Topics:
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> 1. Slide and Cassette printing (Dana Spencer)
> 2. Re: EM question (koellingr <@t> comcast.net)
> 3. Re:counterstain for fast red (Silvina Molinuevo)
> 4. Processor list of cases for Competency (Jimmy Lofton)
> 5. RE: Slide and Cassette printing (Janice Mahoney)
> 6. ?QIHC (Sara Baldwin/mhhcc.org)
> 7. RE: ?QIHC (Walter Benton)
> 8. Second Shift lead position available at CellNetix Labs in
> Seattle (Patrick Laurie)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 15 Nov 2011 09:17:06 -0500
> From: "Dana Spencer" <Dana.Spencer <@t> PCMH.COM>
> Subject: [Histonet] Slide and Cassette printing
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4EC22E11.536C.000A.0 <@t> PCMH.COM>
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> What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes.
>
> Thanks,
> Dana Spencer, CT(ASCP)
> Anatomic Pathology Manager
> Pitt County Memorial Hospital
> Greenville, NC 27834
>
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> ------------------------------
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> Message: 2
> Date: Tue, 15 Nov 2011 14:19:23 +0000 (UTC)
> From: koellingr <@t> comcast.net
> Subject: Re: [Histonet] EM question
> To: Cheryl Crowder <ccrowder <@t> vetmed.lsu.edu>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <331082704.1898578.1321366763290.JavaMail.root <@t> sz0001a.emeryville.ca.mail.comcast.net>
>
> Content-Type: text/plain; charset=utf-8
>
>
>
>
> Cheryl,
>
> Â
>
> Have done some things with ordinary sectioning cryo-electron microscopy but not your specific application. But can go to Cold Harbor Springs Protocols and they can get "Immersion Freezing of Cell Monolayers for Cryo-Electron Tomongraphy".
>
> Â
>
> Even better (more immediate) go here http://www.jove.com/video/1943/electron-cryotomography-of-bacterial-cells  and is a California Institute of Technology (really well produced) video/audio/written explaination of the whole procedure.
>
> Â
>
> Ray
>
> Â
>
> Ray Koelling
>
> PhenoPath Labs
>
> Seattle, WA
>
>
>
> ----- Original Message -----
>
>
>
>
> From: "Cheryl Crowder" <ccrowder <@t> vetmed.lsu.edu>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Monday, November 14, 2011 4:20:35 PM
> Subject: [Histonet] EM question
>
> I am the first to admit I know little to nothing about EM. Â I have a
> researcher who read an article today that said in the method that they had
> taken cells, applied them to copper EM grids and immersed them in liquid
> nitrogen to fix them. Â Has anyone ever heard of this technique? and if you
> have, do you have any directions or references for this technique?
> Cheryl
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> ------------------------------
>
> Message: 3
> Date: Tue, 15 Nov 2011 06:57:20 -0800 (PST)
> From: Silvina Molinuevo <silvinamolinuevo <@t> yahoo.com.ar>
> Subject: [Histonet] Re:counterstain for fast red
> To: "histonet-bounces <@t> lists.utsouthwestern.edu"
> <histonet-bounces <@t> lists.utsouthwestern.edu>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1321369040.38494.YahooMailNeo <@t> web113603.mail.gq1.yahoo.com>
> Content-Type: text/plain; charset=utf-8
>
> Hi Kim! light green ussually looks well with red background.
> before you use it be sure to know how to prepare it!
> best regards, sil
>
> ------------------------------
>
> Message: 4
> Date: Tue, 15 Nov 2011 10:22:08 -0500
> From: "Jimmy Lofton" <loftonjt <@t> holycrosshealth.org>
> Subject: [Histonet] Processor list of cases for Competency
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4EC23D500200005600003F6A <@t> nodcdmg2.no.trinity-health.org>
> Content-Type: text/plain; charset=us-ascii
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> Does anyone in histoland have a list of types of specimens that a histotechnologist who is processing specimens could be used for initial competency.
>
> Thanks,
>
> Jimmy
>
>
> Jimmy Lofton, M.S., HT,CT(ASCP)
> Manager Histology Laboratory
> Holy Cross Hospital
> 1500 Forest Glen Road
> Silver Spring, MD 20910-1484
> 301-754-7353 (Phone)
> 301-754-8563 (Fax)
> loftonjt <@t> holycrosshealth.org
>
>
> Trinity Health MailGate made the following annotations
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> ------------------------------
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> Message: 5
> Date: Tue, 15 Nov 2011 07:02:16 -1000
> From: Janice Mahoney <mamawooo <@t> hotmail.com>
> Subject: RE: [Histonet] Slide and Cassette printing
> To: <dana.spencer <@t> pcmh.com>, histo net
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BLU159-W235BB408595A1FC6EE692ED7C10 <@t> phx.gbl>
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>
> Hi Dana,
> We used the slide labels for the Vantage system without any problems for several years. We labeled at the cutting station and NEVER had a label wash off or smear in about three years. I highly recommend it. You will also see a huge gain in productivity elimination double labeling.
> Hope all is well with you. Call me anytime, it would be great to hear form you.
> Jan Mahoney
> Omaha, NE
>
>
>> Date: Tue, 15 Nov 2011 09:17:06 -0500
>> From: Dana.Spencer <@t> PCMH.COM
>> To: histonet <@t> lists.utsouthwestern.edu
>> Subject: [Histonet] Slide and Cassette printing
>>
>> What hardware are you using for printing slides and cassettes? There are many good systems out there and each have their flaws, but specifically I have heard negative rumblings about the PrintMate and SlideMate Systems from ThermoFisher. Are you using labels at the microtome? I would welcome any feedback or recommendations esp from larger institutions with large volumes.
>>
>> Thanks,
>> Dana Spencer, CT(ASCP)
>> Anatomic Pathology Manager
>> Pitt County Memorial Hospital
>> Greenville, NC 27834
>>
>> ------------------------------------------------------------------------------
>> The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system.
>> ==============================================================================
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>
> ------------------------------
>
> Message: 6
> Date: Tue, 15 Nov 2011 12:33:16 -0500
> From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
> Subject: [Histonet] ?QIHC
> To: "histonet <@t> lists.utsouthwestern.edu "
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
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> Content-Type: text/plain; charset=ISO-8859-1
>
> I WOULD LIKE TO KNOW ALSO
>
> Thanks
> Histology/Cytology Supervisor
> S. Kathy Baldwin, SCT (ASCP)
> Memorial Hospital and Health Care Center
> sbaldwin <@t> mhhcc.org
> Ph 812-996-0210, 0216, Fax 812-996-0232,
> Pager 812-481-0897, Cell 812-887-3357
> Confidential information, Authorized use only.
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 15 Nov 2011 12:44:52 -0500
> From: Walter Benton <wbenton <@t> cua.md>
> Subject: RE: [Histonet] ?QIHC
> To: Sara Baldwin/mhhcc.org <sbaldwin <@t> mhhcc.org>,
> "histonet <@t> lists.utsouthwestern.edu "
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0B8979A204680A42B93A52B486088CD9206C20444F <@t> CUAEXH1.GCU-MD.local>
> Content-Type: text/plain; charset="us-ascii"
>
> I think it is valuable since it allows you to learn things through study and application that you may not have been previously exposed to while preparing for the test. The test is strictly theory now versus the first year when stained slides were submitted and there was zero theory. Given the rapid changes in histology with ISH, IHC, IFs and other molecular based testing I don't see how it could hurt you to earn this credential. I'm not sure how pays is differentiated in the market for this qualification, but I do know that I see more and more jobs, especially those for IHC that require it or it is highly desirable for the position.
>
> Walter Benton HT(ASCP)QIHC
> Histology Supervisor
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 126
> (All Deliveries to Suite 127)
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> wbenton <@t> cua.md
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org [sbaldwin <@t> mhhcc.org]
> Sent: Tuesday, November 15, 2011 12:33 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] ?QIHC
>
> I WOULD LIKE TO KNOW ALSO
>
> Thanks
> Histology/Cytology Supervisor
> S. Kathy Baldwin, SCT (ASCP)
> Memorial Hospital and Health Care Center
> sbaldwin <@t> mhhcc.org
> Ph 812-996-0210, 0216, Fax 812-996-0232,
> Pager 812-481-0897, Cell 812-887-3357
> Confidential information, Authorized use only.
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> ------------------------------
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> Message: 8
> Date: Tue, 15 Nov 2011 09:51:01 -0800
> From: Patrick Laurie <foreightl <@t> gmail.com>
> Subject: [Histonet] Second Shift lead position available at CellNetix
> Labs in Seattle
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAKEyg-0bpszxopyUARhRivDW-fbSmShGNOuRSc4VY4bN+Y7DBg <@t> mail.gmail.com>
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>
> Histonet,
>
>
>
>
>
> *Job Title:* Lead Histotech – 2nd shift
>
> *Department: * Laboratory
>
> *Reports To:* Histology Supervisor
>
> *Shifts: *3:00pm to 11:30PM Monday thru Friday
>
> *SUMMARY*
>
> Under the supervision of the Histology Supervisor, the Lead Histology
> Technician/Technologist, performs with proficiency and understanding, the
> tests and procedures assigned to the Histology Department. The position is
> responsible for grossing, processing, embedding, cutting and staining of
> tissue blocks, accurate reporting of results, quality control, teaching,
> special projects, equipment maintenance and all other duties assigned by
> the Supervisor.
>
> * *
>
> *ESSENTIAL FUNCTIONS*
>
> · Performs both routine and complex special procedures with the
> understanding of tissue structures, techniques, principles, theory and
> instrumentation.
>
> · Able to gross section and dictate descriptions of simple
> specimens, process, embed, cut and stain all types of tissue specimens
> received in the Histology Department.
>
> · Coverslip stained sections, verify that the staining shows
> adequate cellular distinction, label, and distribute to the pathologist
> responsible for reviewing the case.
>
> · Assures specimen labeling requirements are met and necessary
> clinical information is available as needed.
>
> · Assist pathologists with frozen section staining and processing.
>
> · Assists laboratory assistant or performs accessioning of patient
> specimens into laboratory computer system when needed.
>
> · Demonstrates general knowledge of pathological and physiological
> conditions that affect test results and tissue staining.
>
> · Selects and maintains appropriate tissue blocks to serve as
> controls for Special Satins. Maintains all documentation that insures that
> all Special Stain control blocks have been adequately tested. Cuts and
> labels control slides for Histology, Special Stains and IHC.
>
> · Able to process and decal bone specimens.
>
> · Recognize and troubleshoot both routine and complex problems and
> assist other Histotechs and lab assistants with technical problems.
>
> · Monitor quality control results and take immediate and proper
> action when controls are unacceptable. Follow defined procedures with only
> supervisor or pathologist approved modifications or deviations.
>
> · Complete all instrument function verification, maintenance, and
> documents according to procedure in Histology area. Ensure that equipment
> defects and malfunctions are reported and repaired.
>
> · Maintains a neat, clean, and orderly work area. At the end of the
> day’s work; is responsible for the cleaning of his/her microtome, filing
> his/ her cut blocks and cleaning any soiled glassware.
>
> · Responsible for the proper handling and disposal of all
> biohazardous materials and chemically hazardous materials including the
> neutralization or recycling of chemicals before disposal or reuse.
>
> · Cuts slides for IHC testing or other sendouts in conformance with
> procedures and submits them for analysis.
>
> · Takes on additional responsibility for training Laboratory
> personnel including Laboratory Assistants, NRT’s and other Histotechs in
> Histology procedures.
>
> · Maybe be asked to write/ update Operating Procedures.
>
> · Must be able to express himself/ herself effectively both in
> written and verbal communications.
>
> · Assists supervisory staff in monitoring workflow and insuring that
> work/ staining priorities are met for the department.
>
> · Will be asked to put new tests/ stains on line.
>
> · May assist pathologists or physicians in the collection of
> specimens from patients.
>
> · Participates in special projects or other duties as assigned by
> supervisor staff or company.
>
> · Always maintains a pleasant, courteous attitude when answering the
> telephone.
>
> · Conforms to laboratory timekeeping procedures. Keeps unexcused
> absences and late arrivals to a minimum.
>
> · Participates in weekend and holiday schedules as staffing
> requirements dictate. Remains flexible and works a share of overtime or
> different shifts if necessary during staffing shortages or emergencies.
>
> · Participates in continuing education classes and courses. Strongly
> encouraged to keep updated on recent advances in the field of histology and
> to take at least 10 credit hours of continuing education a year.
>
> · Highly encouraged to maintain membership in a professional
> Histology organization.
>
> · Always maintains a safe work environment and attends all safety
> training classes and conforms to all company safety guidelines and
> requirements.
>
> · Conforms to established and new procedures and policies instituted
> by the company.
>
> · Conforms to the Laboratory Dress code and wears appropriate name
> badge at all times.
>
> · Able to accept guidance and constructive criticism. Displays a
> mature and professional attitude, is honest, and has a strong sense of
> responsibility toward patients and clients.
>
> · Able to do all the duties of a HT, Non – Registered
> Histotechnician, Lab Tech II, and Laboratory Assistant within the Histology
> department. During heavy workloads or staffing shortages, the Histology
> Technologist may be required to perform any or all of the duties in these
> positions.
>
> We are seeking an experienced person for a lead position on 2nd shift with
> hours from 3pm to 11:30pm. This is a hands-on position with the Lead
> performing all histo duties in addition to being a trainer and answer
> person for the shift. Five years histotech experience required with at
> least 1 year in a Lead or higher role. Must be certified HT/HTL.
> Please apply via the CellNetix website:
> http://www.cellnetix.com/careers/job-openings
>
> Thanks,
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> plaurie <@t> cellnetix.com
>
>
>
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> plaurie <@t> cellnetix.com
>
>
> ------------------------------
>
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