[Histonet] Thick cryosections
andreahooper <@t> rocketmail.com
Sun Nov 13 09:06:01 CST 2011
Sorry for the late response. I have done immunofluorescence on 50 um cryosections of mouse host tumors and decalcified bone on super frost Plus slides. I melt them to the slide using body heat then dry them at RT for a few hours before storing at -80 deg C. They do not come off the slide. What step do your sections come off the slide? Perhaps you can optimize your protocol to avoid this.
On Oct 11, 2011, at 11:11 AM, "Carmen Maria Garcia Pascual"<Carmen.M.Garcia <@t> uv.es> wrote:
> Good afternoon everyone!
> I have made 50um mice ovarian cryosections and I have used superfrost
> plus slides. But some of them fall of the slides when I perfomed the
> immuno, one colleague has told me that this is because the cuts are too
> thick, and I think she is rigth. I don't know if there is some thing
> that I can make to avoid this, like using another kind of slides...
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