[Histonet] ? on frozen tissue artifacts

[Andrea Hooper]

Sounds to me Definitely more likely to be the freezing process. Do you know their process?

Andrea

On Oct 22, 2011, at 5:05 PM, histopath101 <@t> gmail.com wrote:

> I never said these were muscles. These are tumors removed and frozen the day before and sent to us on dry ice. We know not to use frost-free freezers!
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> On , "Esparza, Sandra" <SEsparza <@t> seton.org> wrote:
>> I have never put my muscles in a -20 we use -70 to -80 freezer and have
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>> not had a problem with artifact. Is your freezer frost free?
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>> Sandra
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>> Sandra Esparza HT(ASCP)QIHC
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>> Lead Technologist
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>> Dell Children's Medical Center of Central Texas
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>> 512-324-0000 x87061
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>> sesparza <@t> seton.org
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>> -----Original Message-----
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>> From: histonet-bounces <@t> lists.utsouthwestern.edu
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>> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
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>> histopath101 <@t> gmail.com
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>> Sent: Friday, October 21, 2011 8:44 AM
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>> To: Histonet <@t> lists.utsouthwestern.edu
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>> Subject: [Histonet] ? on frozen tissue artifacts
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>> I work in a reference lab and some of our frozen sections exhibit severe
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>> freeze artifact (ice crystals). The client claims it's something we're
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>> doing and we say they are not freezing them properly. We receive the
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>> specimens on dry ice and store them in a -20C freezer until we section
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>> them
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>> (no more than 1-2 days later). We never allow the tissues to thaw. My
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>> question: Can freeze artifact occur AFTER properly snap-freezing tissue
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>> or
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>> does this artifact ONLY occur during the initial preezing procedure?
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