[Histonet] Ventana Eber Probes

Bauer, Karen L. Bauer.Karen <@t> mayo.edu
Fri Nov 4 10:16:57 CDT 2011

Hi Andria,

This is our EBER protocol that we are using on our XT... With great

Using the XT ISH Open Probes - Chromogenic procedure...

Enzyme:  ISH Protease 3 for 12 minutes
Probe Auto Dispense:  Then pick the probe that contains EBER
Denature:  60 degrees C  for 4 minutes
Hybridization:  37 degrees C for 1 hour
Stringency Washes
Stringency Wash #1:  47 degrees C for 8 minutes
Stringency Wash #2:  8 minutes
Stringency Wash #3:  8 minutes
Detection Kit
Blue Detection
Since Default is iView Blue+, just put 32 minutes in for the Substrate
Incubation Time
Counterstain:  Red Stain II for 4 minutes

This protocol has worked well for us.  We save our Ultra for routine IPs
throughout the day and only use the XT for ISH runs, since they take

Good Luck,


Karen L. Bauer HTL/HT (ASCP)
Histology Supervisor
Pathology Department
Mayo Clinic Health System in Eau Claire
Phone:  715-838-3205
E-mail:   bauer.karen <@t> mayo.edu
Mayo Clinic Health System
1221 Whipple St.
Eau Claire, WI 54703

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans,
Andria B
Sent: Friday, November 04, 2011 8:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Eber Probes

I am struggling with my EBER probe on both the Ventana Benchmark XT's
and Ultra.  I was hoping that someone could help me out with what you
are currently running with the new probe setup.   Thank you!

A. Harris

(717)544-5457 ~ FAX (if it would be easier to fax the protocol)

This email was sent securely from the LGHealth Email Service

Confidentiality Notice: 
This e-mail message, including any attachments, is for the sole use of
intended recipient(s) and may contain confidential and privileged
Any unauthorized review, use, disclosure or distribution is prohibited.

If you are not the intended recipient, please contact the sender by
reply e-mail and destroy all copies of the original message.

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list