[Histonet] Ventana Eber Probes

[Bauer, Karen L.]

Hi Andria,

This is our EBER protocol that we are using on our XT... With great
success.

Using the XT ISH Open Probes - Chromogenic procedure...

Depar
Enzyme:  ISH Protease 3 for 12 minutes
Probe
HybReady
Probe Auto Dispense:  Then pick the probe that contains EBER
Denature:  60 degrees C  for 4 minutes
Hybridization:  37 degrees C for 1 hour
Stringency Washes
Stringency Wash #1:  47 degrees C for 8 minutes
Stringency Wash #2:  8 minutes
Stringency Wash #3:  8 minutes
Detection Kit
Blue Detection
Since Default is iView Blue+, just put 32 minutes in for the Substrate
Incubation Time
Counterstain:  Red Stain II for 4 minutes


This protocol has worked well for us.  We save our Ultra for routine IPs
throughout the day and only use the XT for ISH runs, since they take
longer.

Good Luck,

Karen

Karen L. Bauer HTL/HT (ASCP)
Histology Supervisor
Pathology Department
Mayo Clinic Health System in Eau Claire
Phone:  715-838-3205
E-mail:   bauer.karen <@t> mayo.edu
___________________________________________
Mayo Clinic Health System
1221 Whipple St.
Eau Claire, WI 54703
www.mayoclinichealthsystem.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans,
Andria B
Sent: Friday, November 04, 2011 8:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Eber Probes

I am struggling with my EBER probe on both the Ventana Benchmark XT's
and Ultra.  I was hoping that someone could help me out with what you
are currently running with the new probe setup.   Thank you!

A. Harris

(717)544-5457 ~ FAX (if it would be easier to fax the protocol)

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