[Histonet] Ventana Eber Probes
Bauer, Karen L.
Bauer.Karen <@t> mayo.edu
Fri Nov 4 10:16:57 CDT 2011
This is our EBER protocol that we are using on our XT... With great
Using the XT ISH Open Probes - Chromogenic procedure...
Enzyme: ISH Protease 3 for 12 minutes
Probe Auto Dispense: Then pick the probe that contains EBER
Denature: 60 degrees C for 4 minutes
Hybridization: 37 degrees C for 1 hour
Stringency Wash #1: 47 degrees C for 8 minutes
Stringency Wash #2: 8 minutes
Stringency Wash #3: 8 minutes
Since Default is iView Blue+, just put 32 minutes in for the Substrate
Counterstain: Red Stain II for 4 minutes
This protocol has worked well for us. We save our Ultra for routine IPs
throughout the day and only use the XT for ISH runs, since they take
Karen L. Bauer HTL/HT (ASCP)
Mayo Clinic Health System in Eau Claire
E-mail: bauer.karen <@t> mayo.edu
Mayo Clinic Health System
1221 Whipple St.
Eau Claire, WI 54703
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans,
Sent: Friday, November 04, 2011 8:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ventana Eber Probes
I am struggling with my EBER probe on both the Ventana Benchmark XT's
and Ultra. I was hoping that someone could help me out with what you
are currently running with the new probe setup. Thank you!
(717)544-5457 ~ FAX (if it would be easier to fax the protocol)
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