[Histonet] Brain sections...

[Weems, Joyce]

And I've always found that letting them air dry overnight before drying in the oven works well too.  j 


Joyce Weems 
Pathology Manager 
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE 
Atlanta, GA 30342 
678-843-7376 - Phone 
678-843-7831 - Fax 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Thursday, November 03, 2011 15:08
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Brain sections...

This works if you are doing traditional specials--never worked out if it affected IHC.  We used it for autopsy brained -called it "double cook"--and you can't scratch the tissue off with a knife after they're handled like this. 
 
Dry your slides in low heat the way you always would.  Then cool completely.  Heat them again til the wax melts and the slide is up to the higher temp (about 10 minutes).  Cool and handle as usual. 
 
Something about the expanding and contracting of going in and out of the heat twice 'snuggles' the tissue onto the slide.  

Cheryl Kerry, HT(ASCP)
Full Staff Inc.
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