[Histonet] DAB Immuno and alcian blue counter staining

[Patsy Ruegg]

Try decreasing the alcian blue concentration and staining time.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniela
Bodemer
Sent: Tuesday, November 01, 2011 8:47 PM
To: Histonet <@t> lists.utsouthwestern.edu
Cc: Susan Lapthorne
Subject: [Histonet] DAB Immuno and alcian blue counter staining

Hi all,

Is it possible to do an immuno with DAB detection and counterstain it
with alcian blue at the end to detect Goblets cells? I've tried it and
the DAB disappears under the staining.

Thanks,

Daniela

______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email 
______________________________________________________________________

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet