[Histonet] Problems with Frozen tissues

[Carmen Maria Garcia Pascual]

Good morning!
I work with ovarie and mice uterus embebbed in oct. I get 8um frozen 
sections and put them un super frost slides, the I leave the slides in 
the fridge until use them. When I am going to perform the 
immunohistochemistry I leave the slides 1 minute at rt to atemperate 
the tissue, then I place the slides 10 minutes in acetone (stored at -
20ºC), whit this you fix the tissue and permeabilize it. with this 
protocol I never loss any sections. If you have any more question just 
write me.
good luck,
Carmen

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Carmen María García Pascual
FIVI/INCLIVA/Facultad de Medicina
Universidad de Valencia
Departamento de P.O.G (Laboratorios)
96.386.40.48
Avd. Blasco Ibañez 17
46010, Valencia
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