[Histonet] Recycled Xylene

Laurie Colbert laurie.colbert <@t> huntingtonhospital.com
Wed May 25 09:07:33 CDT 2011

We process with recycled xylene and have no problems.  We also use it
for staining but use fresh for coverslipping.
Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Marshall, Kimberly K
Sent: Wednesday, May 25, 2011 6:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Recycled Xylene

Hello in Histo land.
  I know it is a subject brought up over and over again but I need to
get the opinion of my fellow Histo techs on processing tissue with
recycled Xylene.  Yes I know it saves money and is better for the earth,
but is the quality of the tissue the same??? Coverslipping and clearing
slides with it I can see being ok, but processing with it??? It is not
100% after recycling.  I could use any thought on the subject.  
Thanks in advance
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