[Histonet] Re: Histonet Digest, Vol 90, Issue 28

Mon May 23 12:41:36 CDT 2011

Dear Miss Margaret Blount:
Congratulations on your retirement!!  May you enjoy good health and many years doing the things you WANT to do!!
God Bless!

-----Original Message-----
From: histonet-request <histonet-request <@t> lists.utsouthwestern.edu>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Mon, May 23, 2011 1:00 pm
Subject: Histonet Digest, Vol 90, Issue 28

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han "Re: Contents of Histonet digest..."

oday's Topics:
   1. Immuno controls (Carrie Disbrow)
  2. This is goodbye (Margaret Blount)
  3. In vitro mouse embryo culture (Carmen Maria Garcia Pascual)
  4. Re: Immuno controls (Rene J Buesa)
  5. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol )
  6. RE: Histonet Digest, Vol 90, Issue 27 (Barone, Carol )
  7. Freezing bath solutions (Scott, Allison D)
  8. p63 (Joanne Clark)

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Message: 1
ate: Sun, 22 May 2011 22:00:05 -0400
rom: Carrie Disbrow <cdisbrow <@t> msn.com>
ubject: [Histonet] Immuno controls
o: <histonet <@t> lists.utsouthwestern.edu>
essage-ID: <SNT117-W41C388EEC39A185424D7E1D0720 <@t> phx.gbl>
ontent-Type: text/plain; charset="iso-8859-1"

i all,
fter consideration I disagree with the consensus and also agree with Curt's 
athologist. More supplies and labor will be  involved as  cutting more controls 
ill be neccessay to meet the requests of the pathologist. The patient tissue is 
ut on two controls slides. One slide is used as the negative control and has 
oth the patient tissue and the known positive control which should both be 
egative after the run. The other slide with the known positive control and the 
atient tissue in question is also stained with the positive control staining 
ositive.
hank you!

------------------------------
Message: 2
ate: Mon, 23 May 2011 09:04:54 +0100
rom: "Margaret Blount" <mab70 <@t> medschl.cam.ac.uk>
ubject: [Histonet] This is goodbye
o: <histonet <@t> lists.utsouthwestern.edu>
essage-ID:
<E2D19A5C4EF4C14FB2B012DB6EEBC36304778BFC <@t> me-mail1.medlan.cam.ac.uk>
ontent-Type: text/plain;	charset="US-ASCII"
To everyone in histoland,

This is my last full week at work. From next Tuesday I will be retiring
rom my job at Cambridge University. I have thoroughly enjoyed my chosen
rofession as a research technician specialising in histology. I realise
hat because I have always worked in research my experience is somewhat
imited: I haven't been asked for the same range of techniques that you
ind in a routine clinical lab. However, I have enjoyed my work and have
earnt and devised some techniques for a range of interesting samples.
ow it's time to learn something new! 

I hope to spend more time with my lace pillow and learn to do it
roperly, maybe learn to do Northamptonshire lace which I believe is a
ariation on Bucks point. (I come originally from Northants) I have had
 go at Bedfordshire and will learn more, it is very pretty. I also plan
o travel; my next trip is to New Zealand in November. 

I won't go on. I just want to say this: You are all great guys and
irls; you share your experience and friendship with great generosity,
hich I have always felt is what a scientist should do. Thank you for
our comradeship and help over the years. Maybe I'll get to meet some of
ou by chance during my travels.

Best wishes to you all and a great big Thank You,

From

Margaret

Miss Margaret Blount
Histology Manager
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ

Tel 01223 769061/336079

------------------------------
Message: 3
ate: Mon, 23 May 2011 10:35:20 +0200 (CEST)
rom: "Carmen Maria Garcia Pascual"<Carmen.M.Garcia <@t> uv.es>
ubject: [Histonet] In vitro mouse embryo culture
o: histonet <@t> lists.utsouthwestern.edu
essage-ID: <6395165382carmaga6 <@t> uv.es>
ontent-Type: text/plain;	charset="ISO-8859-1"

Good Moorning to everyone:
I am Carmen Garcia a PhD student from Valencia, we are know interesting 
n make in vitro culture of mouse embryos to do toxicity tests and in 
ur lab we have not experience. someone can give me some advise? or 
ecommend me some bibliography?
ow long can I have the embryos in culture?
hank you so much!!!
Best regards,
armen

------------------------------
Message: 4
ate: Mon, 23 May 2011 07:48:50 -0700 (PDT)
rom: Rene J Buesa <rjbuesa <@t> yahoo.com>
ubject: Re: [Histonet] Immuno controls
o: Carrie Disbrow <cdisbrow <@t> msn.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
essage-ID: <87688.31423.qm <@t> web65714.mail.ac4.yahoo.com>
ontent-Type: text/plain; charset=iso-8859-1
Also Sprach Zarathustra!!!!
en� J.
From: Carrie Disbrow <cdisbrow <@t> msn.com>
o: histonet <@t> lists.utsouthwestern.edu
ent: Sunday, May 22, 2011 10:00 PM
ubject: [Histonet] Immuno controls

i all,
fter consideration I disagree with the consensus and also agree with Curt's 
athologist. More supplies and labor will be� involved as� cutting more controls 
ill be neccessay to meet the requests of the pathologist. The patient tissue is 
ut on two controls slides. One slide is used as the negative control and has 
oth the patient tissue and the known positive control which should both be 
egative after the run. The other slide with the known positive control and the 
atient tissue in question is also stained with the positive control staining 
ositive.
hank you!
��� ��� ��� � ��� ��� � _______________________________________________
istonet mailing list
istonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
ate: Mon, 23 May 2011 11:02:16 -0400
rom: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27
o: <histonet <@t> lists.utsouthwestern.edu>
essage-ID:
<DA71F2870E979549818D426EFD7A37AD855F6A <@t> wlmmsx02.nemours.org>
ontent-Type: text/plain;	charset="us-ascii"

-----Original Message-----
rom: histonet-bounces <@t> lists.utsouthwestern.edu
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
istonet-request <@t> lists.utsouthwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histonet <@t> lists.utsouthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send Histonet mailing list submissions to

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r, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than
Re: Contents of Histonet digest..."

oday's Topics:
   1. to Rene J Buesa (Maxim Peshkov)

---------------------------------------------------------------------
Message: 1
ate: Sun, 22 May 2011 13:43:07 +0400
rom: Maxim Peshkov <Maxim_71 <@t> mail.ru>
ubject: [Histonet] to Rene J Buesa
o: Rene J Buesa <rjbuesa <@t> yahoo.com>
c: histonet <@t> lists.utsouthwestern.edu
essage-ID: <1585823684.20110522134307 <@t> mail.ru>
ontent-Type: text/plain; charset=windows-1251
I am sorry, that uses histonet for personal goals.
Dear Rene!
ast 3 my answers for you are returned back to me.
our e-mails I got successfully.
upport service of mail.ru wrote that problem is at yahoo.com server.
lease check your postmaster service.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A,
47909,
aganrog,
ussia.
Sincerely,
axim.
                      mailto:Maxim_71 <@t> mail.ru

-----------------------------
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End of Histonet Digest, Vol 90, Issue 27
***************************************

------------------------------
Message: 6
ate: Mon, 23 May 2011 11:15:34 -0400
rom: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
ubject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27
o: <histonet <@t> lists.utsouthwestern.edu>
essage-ID:
<DA71F2870E979549818D426EFD7A37AD855F6B <@t> wlmmsx02.nemours.org>
ontent-Type: text/plain;	charset="us-ascii"
Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, that
ad already been in formailin two weeks (was FFPE).  Normally would do
n a frozen 1:40? ....We have background everywhwere on first
un...suggestions? Neg is also shining??? Crazy researcher's! Got to
ove them...when will they learn to ask first?
We are diluting primary in plan two, and will stain over-night at 4
egrees....but, this thing is lit-up like the SUN!
 was expecting low signal? Go figure? 

----Original Message-----
rom: histonet-bounces <@t> lists.utsouthwestern.edu
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
istonet-request <@t> lists.utsouthwestern.edu
ent: Sunday, May 22, 2011 1:01 PM
o: histonet <@t> lists.utsouthwestern.edu
ubject: Histonet Digest, Vol 90, Issue 27
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
r, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than
Re: Contents of Histonet digest..."

oday's Topics:
   1. to Rene J Buesa (Maxim Peshkov)

---------------------------------------------------------------------
Message: 1
ate: Sun, 22 May 2011 13:43:07 +0400
rom: Maxim Peshkov <Maxim_71 <@t> mail.ru>
ubject: [Histonet] to Rene J Buesa
o: Rene J Buesa <rjbuesa <@t> yahoo.com>
c: histonet <@t> lists.utsouthwestern.edu
essage-ID: <1585823684.20110522134307 <@t> mail.ru>
ontent-Type: text/plain; charset=windows-1251
I am sorry, that uses histonet for personal goals.
Dear Rene!
ast 3 my answers for you are returned back to me.
our e-mails I got successfully.
upport service of mail.ru wrote that problem is at yahoo.com server.
lease check your postmaster service.
Here is my post-address:
axim Peshkov,
l. Mikhailovskaya, 90-A,
47909,
aganrog,
ussia.
Sincerely,
axim.
                      mailto:Maxim_71 <@t> mail.ru

-----------------------------
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istonet mailing list
istonet <@t> lists.utsouthwestern.edu
ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 90, Issue 27
***************************************

------------------------------
Message: 7
ate: Mon, 23 May 2011 11:22:24 -0500
rom: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
ubject: [Histonet] Freezing bath solutions
o: <histonet <@t> lists.utsouthwestern.edu>
essage-ID:
<1872B4A455B7974391609AD8034C79FC026DFDD9 <@t> LBEXCH01.hchd.local>
ontent-Type: text/plain; charset="us-ascii"
Hello to all in histoland.  What type of solutions are being used if any
o freezze your chucks for frozen sections.  Our sister hospital is
sing methyl butane and there was a flash fire last week.  We use the
ame solution.  Any suggestions will be greatly appreciated.
Allison Scott HT(ASCP)
istology Supervisor
BJ Hospital
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Message: 8
ate: Mon, 23 May 2011 16:31:08 +0000
rom: Joanne Clark <jclark <@t> pcnm.com>
ubject: [Histonet] p63
o: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
essage-ID:
<0494A7D4E8CC254EA2FB81464982E3786DCA72 <@t> S10MAILD001N1.SH10.lan>
ontent-Type: text/plain; charset="us-ascii"
I need to find a source for p63 antibody.  Where does everyone purchase theirs 
rom?
Joanne Clark,HT
athology Consultants of New Mexico

------------------------------
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End of Histonet Digest, Vol 90, Issue 28
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