[Histonet] Ventana E-cadherin problem

[Dessoye, Michael J]

Hello Histonet,
 
We've been having a recurring problem with E-cadherin from Ventana on Benchmark Ultra.  It's spanned two different lots of antibody.  Our tissues are suddenly falling off the slides.  Negative controls are ok, just seems to be after antibody treatment.  The protocol hasn't changed, it's the manufacturer recommended protocol.  It also seems to happen to various tissue types and fixation times.  We've tried drying the slides longer and it doesn't seem to help.  Anyone else having problems with this antibody?
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdessoye <@t> wvhcs.org <mailto:mjdessoye <@t> wvhcs.org>  |
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 
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