[Histonet] nuclear bubbling

Patsy Ruegg pruegg <@t> ihctech.net
Fri May 13 11:59:43 CDT 2011

This is most likely caused by inadequate fixation in formalin which would
protect the tissues from tissue processing.  With such fast turn around
times we are seeing this more and more.  Put formalin on your tissue
processor and use any extra time you may have up front in formalin rather
than tissue processing.  Ideally it takes 24 hrs to properly fix tissues so
they are not damaged by processing and embedding in paraffin, but nobody
fixes that long anymore.


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, May 12, 2011 1:41 PM
To: Johnson, Nacaela; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] nuclear bubbling

Heat by itself is not the cause, but the heat applied to sections that have
not been properly drained and still have water left between them and the
slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.

From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling

Has anyone one had problems with nuclear bubbling?  I have read about
two different causes.  (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a high temp (70 degrees C or above) while
drying.  The latter is definitely not happening, but I do use formalin.
I am in the process of changing the type of formalin that is used during
collection.  Does anyone have any other suggestions?  Has this problem
occurred for any other reason than I have already pointed out?  It seems
to be worse in the surgical needle biopsies than in the bone marrow


Nacaela Johnson, B.S. HTL (ASCP)CM


KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  Nacaela.Johnson <@t> USOncology.com

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