[Histonet] nuclear bubbling

[Goins, Tresa]

The best tip I ever received was from a gentleman in Australia who suggested adding a very small - so small it would appear to be useless - amount of Tween 20 to the floatation water bath.  The entrapment of water is eliminated - no more blebs of water at the base of the sections.  Works like magic.


Tresa Goins
Histopathology Supervisor
Department of Livestock
Bozeman, Montana

  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela
Sent: Thursday, May 12, 2011 2:25 PM
To: Rene J Buesa; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling

Another individual called me and commented further, which clarified your statement.  It is actually poor lab practice to combine the drying/baking.  These steps should really be done separately so as to not "trap" the water under the tissue.  I have noticed bubbles of water on the bottom of my slides that are encapsulated in wax.  I assume this is also happening under the tissue as well, therefore affecting the nuclear morphology.  I had been taught to speed up the process by placing them in the oven directly after cutting.  Decreasing the TAT was all they really cared about.  Fortunately where I work now chooses quality over quantity and I can adjust my protocol to get rid of the artifact.  Thank you all for your comments and clarity.


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Nacaela
Sent: Thursday, May 12, 2011 2:50 PM
To: Rene J Buesa; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling

So there really isn't a point in using an oven to dry the slides?  I currently dry my slides for an hour at 55-60 degrees C. 


Thanks,
 
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, May 12, 2011 2:41 PM
To: Johnson, Nacaela; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] nuclear bubbling

Nacaela: 
Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.

From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling

Has anyone one had problems with nuclear bubbling?  I have read about two different causes.  (1) Formalin itself is known to cause the issue and (2) heating the tissue at a high temp (70 degrees C or above) while drying.  The latter is definitely not happening, but I do use formalin.
I am in the process of changing the type of formalin that is used during collection.  Does anyone have any other suggestions?  Has this problem occurred for any other reason than I have already pointed out?  It seems to be worse in the surgical needle biopsies than in the bone marrow biopsies.


Thanks,



Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  Nacaela.Johnson <@t> USOncology.com


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