[Histonet] nuclear bubbling

[Rene J Buesa]

Nacaela: 
Heat by itself is not the cause, but the heat applied to sections that have not been properly drained and still have water left between them and the slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
René J.

From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling

Has anyone one had problems with nuclear bubbling?  I have read about
two different causes.  (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a high temp (70 degrees C or above) while
drying.  The latter is definitely not happening, but I do use formalin.
I am in the process of changing the type of formalin that is used during
collection.  Does anyone have any other suggestions?  Has this problem
occurred for any other reason than I have already pointed out?  It seems
to be worse in the surgical needle biopsies than in the bone marrow
biopsies.


Thanks,



Nacaela Johnson, B.S. HTL (ASCP)CM

Histotechnologist

KCCC Pathology

12000 110th St., Ste. 400

Overland Park, KS 66210

Office:  913-234-0576

Fax:  913-433-7639

Email:  Nacaela.Johnson <@t> USOncology.com


</pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet