[Histonet] preference: fat stains

[Tony Henwood]

Definitely go with Oil Red O rather than Osmium.
Osmium tetroxide reacts with unsaturated lipids whereas ORO colours neutral lipids.

You can do an ORO on formalin fixed tissue (frozen sections).
You could also look at the Sudan Black techniques.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michelle Perrins
Sent: Friday, 6 May 2011 11:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] preference: fat stains

Hello
 
I would like your views on which provides the better result regarding fat stains.
1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue
 
I know that osmium is nasty to work with but besides that does it produce a better result?
 
Thanks
 
Michelle 
 
 
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine
Faculty Health Sciences
University of Cape Town
 
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: Michelle.Perrins <@t> uct.ac.za


 

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