[Histonet] preference: fat stains

Liz Chlipala liz <@t> premierlab.com
Fri May 6 11:37:35 CDT 2011


Michelle

We use osmium all of the time, we find it works better for image
analysis than the Oil Red O.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michelle
Perrins
Sent: Friday, May 06, 2011 7:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] preference: fat stains

Hello
 
I would like your views on which provides the better result regarding
fat stains.
1. fat stains (Oil red O, etc) on frozen sections
2. osmium tetroxide on formalin fixed tissue
 
I know that osmium is nasty to work with but besides that does it
produce a better result?
 
Thanks
 
Michelle 
 
 
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine 
Faculty Health Sciences
University of Cape Town
 
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: Michelle.Perrins <@t> uct.ac.za


 

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