[Histonet] preference: fat stains

[Liz Chlipala]

Michelle

We use osmium all of the time, we find it works better for image
analysis than the Oil Red O.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michelle
Perrins
Sent: Friday, May 06, 2011 7:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] preference: fat stains

Hello
 
I would like your views on which provides the better result regarding
fat stains.
1. fat stains (Oil red O, etc) on frozen sections
2. osmium tetroxide on formalin fixed tissue
 
I know that osmium is nasty to work with but besides that does it
produce a better result?
 
Thanks
 
Michelle 
 
 
Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine 
Faculty Health Sciences
University of Cape Town
 
tel: +27 21 406 6001
fax: +27 21 448 1249
Email: Michelle.Perrins <@t> uct.ac.za


 

###
UNIVERSITY OF CAPE TOWN 

This e-mail is subject to the UCT ICT policies and e-mail disclaimer
published on our website at
http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from
+27 21 650 9111. This e-mail is intended only for the person(s) to whom
it is addressed. If the e-mail has reached you in error, please notify
the author. If you are not the intended recipient of the e-mail you may
not use, disclose, copy, redirect or print the content. If this e-mail
is not related to the business of UCT it is sent by the sender in the
sender's individual capacity.

###
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet