[Histonet] Poor contrast in H&E stain

Heather R hrumbut <@t> yahoo.com
Wed May 4 21:33:17 CDT 2011


I am in the process of setting up stain run for our new stainer and am having a problem getting "crisp" contrast in our H&E.  I have adjusted times with little improvement. The stain looks "muddy" I guess. The pathologist says it's the contrast.  We are doing skin biopsies. Using Xylene, Gills, richard allen clarifier , doesnt seem like this should be too hard, but it's getting frustrating.
Thanks
HR


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